Mechanism of Eriochloa villosa (Thunb.) Kunth Resistance to Nicosulfuron

Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due to continuous and extensive application. We identified a biotype showing resistance to ALS inhibitor nicosulfuron with a resistant index 13.83, but without any target spot mutation....

Full description

Saved in:
Bibliographic Details
Published in:Agronomy (Basel) 2024-10, Vol.14 (10), p.2210
Main Authors: Guo, Jing, Xu, Zeqian, Jiao, Ting, Gao, Hong, Wang, Yuechao, Zhang, Liguo, Li, Mukai, Liu, Xiaomin, Yan, Chunxiu, Han, Yujun
Format: Article
Language:English
Subjects:
ABC transporter
Agricultural production
Alanine
Analysis
Aspartate
Biosynthesis
Corn
Crops
E. villosa
Enzymes
Eriochloa villosa
Food security
Food supply
Gene expression
Gene sequencing
Genes
Genetic aspects
Genetic transcription
Glutathione
Herbicide resistance
Herbicides
Impact resistance
Leaves
metabolic resistance
Metabolism
nicosulfuron
Pesticides
RNA
RNA sequencing
Seeds
Target spot
transcriptome
Transcriptomes
Weeds
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due to continuous and extensive application. We identified a biotype showing resistance to ALS inhibitor nicosulfuron with a resistant index 13.83, but without any target spot mutation. Herein, transcriptome sequencing was used to analyze the differences in gene expression at the transcriptional level between nicosulfuron-resistant E. villosa HEK-40 varieties and sensitive E. villosa HEK-15 varieties. The resistant and sensitive varieties comparison revealed 9931 DEGs after nicosulfuron application, of which 5426 and 4505 genes were up-regulated and down-regulated, respectively. Some contigs related to metabolic resistance were identified based on differential expression via RNA-Seq, which includes ABC transporters (ko02010), glucosinolate biosynthesis (ko00966), 2-oxocarboxylic acid metabolism (ko01210), alanine, aspartate, and glutamate metabolism pathways (ko00250). Seven CYP450 genes, four GST genes, ten ABC transporter genes, and two GT genes related to metabolic resistance were identified. The 10 candidate genes screened were validated using q-PCR. This validation indicates that activities associated with P450 enzymes, ABC transporters, and glutathione S-transferases (GST) may play a role in conferring resistance, which is important for reducing the impact of weeds on corn fields and ensuring food security.
ISSN:2073-4395
2073-4395
DOI:10.3390/agronomy14102210