Evaluation of the Nature of Collagen Fibers in KCOT, Dentigerous Cyst and Ameloblastoma using Picrosirius Red Stain - A Comparative Study

Reciprocal interaction between dental epithelium and mesenchyme is thought to be crucial for normal odontogenesis. Thus, the mesenchymal influence of the fibrous capsules may play an important role in the maintenance of epithelial expression. Collagen is the major component of the extracellular matr...

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Bibliographic Details
Published in:Journal of clinical and diagnostic research 2015-11, Vol.9 (11), p.ZC01-ZC04
Main Authors: Raj, Yukti, Sekhar, Manne Srinivas Muni, Shylaja, Sanjeevareddygari, Bhavani, Sangala Nagendra, Ramanand, Oruganti Venkata, Patha, Spandana, Reddy, Sharath Kumar, Rani, Akula Sandhya
Format: Article
Language:English
Subjects:
collagen fibers
Dentistry Section
odontogenic cysts and tumours
picrosirius red
polarizing microscopy
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Summary:Reciprocal interaction between dental epithelium and mesenchyme is thought to be crucial for normal odontogenesis. Thus, the mesenchymal influence of the fibrous capsules may play an important role in the maintenance of epithelial expression. Collagen is the major component of the extracellular matrix and possibly there is an alteration in the nature and structure of collagen in various pathological conditions. Studies by polarizing microscopy have also shown that there is a difference in collagen and probably these differences may play a role in their biologic behaviour. The purpose of this study was to evaluate the nature of collagen fibers in keratocystic odontogenic tumour (KCOT), dentigerous cyst (DC), unicystic ameloblastoma (UA) and solid/multicystic ameloblastoma (SMA) and correlating this with their biological behaviour. Five diagnosed cases each of UA, SMA, KCOT and DC were taken and stained using Picrosirius red stain kit and evaluated using a polarizing microscope. Chi-square test was used to analyse the results. Collagen fibers in dentigerous cysts showed predominant yellowish-red birefringence and fibers in KCOT and ameloblastomas showed a predominantly greenish-yellow birefringence. Hence, our study suggests that the nature and character of collagen fibers may influence the clinical behaviour of the lesion. Since ours is a pilot study, to corroborate our view, studies with larger sample size are required to substantiate the results.
ISSN:2249-782X
0973-709X
DOI:10.7860/JCDR/2015/14154.6708