A bioactive peptide amidating enzyme is required for ciliogenesis

The pathways controlling cilium biogenesis in different cell types have not been fully elucidated. We recently identified peptidylglycine α-amidating monooxygenase (PAM), an enzyme required for generating amidated bioactive signaling peptides, in and mammalian cilia. Here, we show that PAM is requir...

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Bibliographic Details
Published in:eLife 2017-05, Vol.6
Main Authors: Kumar, Dhivya, Strenkert, Daniela, Patel-King, Ramila S, Leonard, Michael T, Merchant, Sabeeha S, Mains, Richard E, King, Stephen M, Eipper, Betty A
Format: Article
Language:English
Subjects:
amidation
Animals
Axonemes
Biosynthesis
Cell Biology
chlamdyomonas
Chlamydomonas
Chlamydomonas - enzymology
Cilia
Cilia - metabolism
Embryos
Enzymes
Gene expression
Gene Knockdown Techniques
Genes
golgi
Golgi apparatus
Mammals
Mice - embryology
Mice, Knockout
Mixed Function Oxygenases - genetics
Mixed Function Oxygenases - metabolism
Monooxygenase
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Organelle Biogenesis
Peptides
peptidylglycine alpha-amidating monooxygenase
Phosphatase
planaria
Planarians - enzymology
Proteins
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Summary:The pathways controlling cilium biogenesis in different cell types have not been fully elucidated. We recently identified peptidylglycine α-amidating monooxygenase (PAM), an enzyme required for generating amidated bioactive signaling peptides, in and mammalian cilia. Here, we show that PAM is required for the normal assembly of motile and primary cilia in , planaria and mice. PAM knockdown lines failed to assemble cilia beyond the transition zone, had abnormal Golgi architecture and altered levels of cilia assembly components. Decreased PAM gene expression reduced motile ciliary density on the ventral surface of planaria and resulted in the appearance of cytosolic axonemes lacking a ciliary membrane. The architecture of primary cilia on neuroepithelial cells in mouse embryos was also aberrant. Our data suggest that PAM activity and alterations in post-Golgi trafficking contribute to the observed ciliogenesis defects and provide an unanticipated, highly conserved link between PAM, amidation and ciliary assembly.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.25728