Identification of Differentially Expressed Genes in Resistant Tetraploid Wheat ( Triticum turgidum ) under Sitobion avenae (F.) Infestation

The grain aphid (Fabricius) is one of the most destructive pests of wheat ( ). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to this pest. The present study was carried out to investigate the potent...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-05, Vol.23 (11), p.6012
Main Authors: Liu, Xinlun, Kou, Xudan, Bai, Shichao, Luo, Yufeng, Wang, Zhenyu, Xie, Lincai, Deng, Pingchuan, Zhang, Hong, Wang, Changyou, Wang, Yajuan, Zhao, Jixin, Ji, Wanquan
Format: Article
Language:English
Subjects:
Antibiosis
aphid resistance
Cultivars
Enzymes
Expressed sequence tags
Fecundity
Gene expression
Genes
Genomes
Germplasm
High resistance
Hybridization
Infestation
Insects
Jasmonic acid
Kinases
Localization
Metabolism
Ontology
Oxidative stress
Pest resistance
Pests
Photosynthesis
Proteins
Salicylic acid
Seedlings
Signal transduction
Sitobion avenae
suppression subtractive hybridization
tetraploid wheat
Triticum turgidum
Wheat
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Summary:The grain aphid (Fabricius) is one of the most destructive pests of wheat ( ). Deployment of resistant wheat germplasm appears as an excellent solution for this problem. Elite bread wheat cultivars only have limited resistance to this pest. The present study was carried out to investigate the potential of the tetraploid wheat ( ) variety Lanmai, which showed high resistance to at both seedling and adult plant stages, as a source of resistance genes. Based on apterous adult aphids' fecundity tests and choice bioassays, Lanmai has been shown to display antixenosis and antibiosis. Suppression subtractive hybridization (SSH) was employed to identify and isolate the putative candidate defense genes in Lanmai against infestation. A total of 134 expressed sequence tags (ESTs) were identified and categorized based on their putative functions. RT-qPCR analysis of 30 selected genes confirmed their differential expression over time between the resistant wheat variety Lanmai and susceptible wheat variety Polan305 during infestation. There were 11 genes related to the photosynthesis process, and only 3 genes showed higher expression in Lanmai than in Polan305 after infestation. Gene expression analysis also revealed that Lanmai played a critical role in salicylic acid and jasmonic acid pathways after infestation. This study provided further insights into the role of defense signaling networks in wheat resistance to and indicates that the resistant tetraploid wheat variety Lanmai may provide a valuable resource for aphid tolerance improvement in wheat.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23116012