Extraction of Opuntia dillenii Haw. Polysaccharides and Their Antioxidant Activities

Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactus Haw. (ODP). Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extrac...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2016-11, Vol.21 (12), p.1612-1612
Main Authors: Li, Heng, Yuan, Qingxia, Zhou, Xianjiao, Zeng, Fuhua, Lu, Xiangyang
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants - chemistry
antioxidation
Biphenyl Compounds - chemistry
characterization
Chromatography
extraction
Hydroxyl Radical - chemistry
Molecular weight
Optimization
Opuntia
Opuntia - chemistry
Opuntia dillenii Haw
Picrates - chemistry
Plant Extracts - chemistry
polysaccharide
Polysaccharides - chemistry
Raw materials
Superoxides - chemistry
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Summary:Use of natural polysaccharides in medicine and food has wide interest in research. In this study, we extracted and purified some polysaccharides from cactus Haw. (ODP). Some preliminary functions of these products were characterized. Under the optimal purification conditions, the yield of ODP extracted from the 2-4 month-old Haw. (T-ODP) was 30.60% ± 0.40%, higher than that of ODP from the 5-10 month-old materials (O-ODP) (18.97% ± 0.58%). The extracted ODP was purified by DEAE sepharose fast flow anion exchange and Sephacryl S-400 chromatography with four fractions obtained (ODP-Ia, ODP-Ib, ODP-IIa and ODP-IIb). Analysis with UV-vis chromatography indicated that ODP-Ia and ODP-IIa were relatively homogeneous molecules with a molecular weight of 339 kD and 943 kD, respectively. Results of infrared spectroscopy indicated that ODP, ODP-Ia, and ODP-IIa were acidic polysaccharides. Further, the antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, hydroxyl radicals, and superoxide radical in vitro demonstrated that the T-ODP exhibited higher antioxidant activity than the O-ODP, and the purified fraction (ODP-Ia) was superior to the ODP. These results will offer a theoretical basis for further research on the structure-function relationship of ODP and the rational utilization of Haw.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules21121612