Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy

Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen o...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors (Basel) 2023-08, Vol.13 (8), p.789
Main Authors: Gigli, Lucia, Braidotti, Nicoletta, Lima, Maria Augusta do R. B. F, Ciubotaru, Catalin Dacian, Cojoc, Dan
Format: Article
Language:English
Subjects:
Analysis
Antibiotics
Bacteria
bacteria detection
Blood
Cancer
Care and treatment
Cell culture
Cells
Cellular biology
Crystals
Diagnosis
digital holographic microscopy
Dosage and administration
Epithelial cells
Epithelium
Erythrocytes
Flow cytometry
Frames per second
Holography
Infectious diseases
Labels
Leukocytes
Medical prognosis
Microscopy
Sample preparation
sample screening
Urinary tract
urinary tract infection
Urinary tract infections
Urine
urine analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen out the negative samples. We propose a digital holographic microscopy (DHM) method to inspect fresh urine samples flowing in a glass capillary for 3 min, recording holograms at 2 frames per second. After digital reconstruction, bacteria, white and red blood cells, epithelial cells and crystals were identified and counted, and the samples were classified as negative or positive according to clinical cutoff values. Taking the CPT as reference, we processed 180 urine samples and compared the results with those of urine flow cytometry (UFC). Using standard evaluation metrics for our screening test, we found a similar performance for DHM and UFC, indicating DHM as a suitable and fast screening technique retaining several advantages. As a benefit of DHM, the technique is label-free and does not require sample preparation. Moreover, the phase and amplitude images of the cells and other particles present in urine are digitally recorded and can serve for further investigation afterwards.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13080789