Fast Determination of Rutin on a Biosensor Made Using a Layered Double Hydroxide Nanocomposite Modified Electrode

In this study, a nanocomposite of LDH/graphene/polyaniline/gold (LDH/rGO/PANI/Au) was synthesized and characterized. The results of characterization showed that the composite material preserved the layered structure of LDH. The composite was dropped onto the glassy carbon electrode and laccase was t...

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Bibliographic Details
Published in:Biosensors (Basel) 2024-01, Vol.14 (1), p.18
Main Authors: Liu, Yuge, Li, Zhiguo, Chen, Weizhen, Feng, Xiaomiao
Format: Article
Language:English
Subjects:
Analysis
Biosensing Techniques
biosensor
Biosensors
Carbon
Chemical synthesis
composite
Composite materials
Electrochemistry
Electrodes
Electron transfer
Enzymes
Flavonoids
Glassy carbon
Graphene
Hydroxides
Laccase
LDH/graphene/polyaniline/gold
Liquid chromatography
Methods
Nanocomposites
Nanoparticles
Polyanilines
Polymers
Polyphenols
Properties
Rutin
Sensors
Signal to noise ratio
Structure
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Summary:In this study, a nanocomposite of LDH/graphene/polyaniline/gold (LDH/rGO/PANI/Au) was synthesized and characterized. The results of characterization showed that the composite material preserved the layered structure of LDH. The composite was dropped onto the glassy carbon electrode and laccase was then immobilized. Electrochemical tests showed that the composite could accelerate the electron transfer between the enzyme and the electrode. The composite/laccase showed an obvious response to rutin and the optimal detection conditions were discussed. The oxidative peak current of the biosensor constructed using the modified electrode was negatively correlated with rutin in the range of 0.05-4 μg/mL. The detection limit was 0.0017 μg/mL at a signal-to-noise ratio of 3. This biosensor of rutin also possessed high sensitivity, excellent anti-interference ability, and stability. The contents of rutin in tablets, first determined using HPLC, were also detected using the sensor constructed in this research as an application, and the results were acceptable. This research here provides a facile way for the fast detection of rutin in real samples.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios14010018