Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein

The re-emergence of the mpox pandemic poses considerable challenges to human health and societal development. There is an urgent need for effective prevention and treatment strategies against the mpox virus (MPXV). In this study, we focused on the A35R protein and created a chimeric A35R-Fc protein...

Full description

Saved in:
Bibliographic Details
Published in:Viruses 2025-01, Vol.17 (1), p.116
Main Authors: Bai, Shimeng, Cui, Yanxin, Liao, Qibin, Yi, Hongyang, Liao, Zhonghui, Zhang, Gengwei, Wu, Fenfang, Lu, Hongzhou
Format: Article
Language:English
Subjects:
A35R protein
Adjuvants
Animals
Antibodies
Antibodies, Neutralizing - blood
Antibodies, Neutralizing - immunology
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antibody Affinity
Antigens
Ethics
Fc fusion protein
Fc receptors
Female
Global health
Humans
Immunization
Immunogenicity
Immunoglobulin Fc Fragments - genetics
Immunoglobulin Fc Fragments - immunology
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin G - immunology
Laboratory animals
Mice
Mice, Inbred BALB C
Mpox
mpox virus
MPXV
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - prevention & control
Orthomyxoviridae Infections - virology
Proteins
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Vaccines
Viruses
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The re-emergence of the mpox pandemic poses considerable challenges to human health and societal development. There is an urgent need for effective prevention and treatment strategies against the mpox virus (MPXV). In this study, we focused on the A35R protein and created a chimeric A35R-Fc protein by fusing the Fc region of IgG to its C-terminal. We then assessed its reactivity with A35R-specific antibodies and human convalescent plasma, as well as its immunogenicity. Our findings indicate that the A35R-Fc protein significantly enhances affinity to A35R antibodies compared to the commercially available A35R protein and exhibits considerable reactivity to human plasma. Additionally, mice immunized with A35R-Fc exhibited increased neutralizing antibody titers against the live MPXV. These results support the potential of Fc domain chimeric antigens as a strategy to enhance the efficacy of subunit vaccines targeting the MPXV.
ISSN:1999-4915
1999-4915
DOI:10.3390/v17010116