SpAD Biofunctionalized Cellulose Acetate Scaffolds Inhibit Staphylococcus aureus Adherence in a Coordinating Function with the von Willebrand A1 Domain (vWF A1)

is one of the major pathogens causing and spreading hospital acquired infections. Since it is highly resistant to new generation antibiotics, novel strategies have to be developed such as the construction of biofunctionalized non-adherent surfaces that will prevent its tethering and subsequent sprea...

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Bibliographic Details
Published in:Journal of functional biomaterials 2022-02, Vol.13 (1), p.21
Main Authors: Pendas, Stefanos, Asiminas, Antonis, Katranidis, Alexandros, Tsioptsias, Costas, Pitou, Maria, Papadopoulos, Georgios, Choli-Papadopoulou, Theodora
Format: Article
Language:English
Subjects:
Acetic acid
antibacterial properties
Antibiotics
Biotin
Blood plasma
Cellulose acetate
cellulose acetate scaffolds
Cloning
E coli
Glycerol
Hemostasis
Hemostatics
Hospitals
Implants
Medical equipment
Nosocomial infections
Polymers
Protein A
Proteins
S. aureus protein A
Scaffolds
Scanning electron microscopy
Staphylococcus aureus
Staphylococcus infections
Streptavidin
Surgical implants
Tethering
Ultrasonic imaging
Von Willebrand factor
von Willebrand factor A1
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Summary:is one of the major pathogens causing and spreading hospital acquired infections. Since it is highly resistant to new generation antibiotics, novel strategies have to be developed such as the construction of biofunctionalized non-adherent surfaces that will prevent its tethering and subsequent spread in the hospital environment. In this frame, the domain D of protein A (SpAD) of has been immobilized onto cellulose acetate scaffolds by using the streptavidin/biotin interaction, in order to study its interaction with the A1 domain of von Willebrand factor (vWF A1), a protein essential for hemostasis, found in human plasma. Subsequently, the biofunctionalized cellulose acetate scaffolds were incubated with in the presence and absence of vWF A1 at different time periods and their potential to inhibit growth was studied with scanning electron microscopy (SEM). The SpAD biofunctionalized scaffolds perceptibly ameliorated the non-adherent properties of the material, and in particular, the interaction between SpAD and vWF A1 effectively inhibited the growth of . Thus, the exhibition of significant non-adherent properties of scaffolds addresses their potential use for covering medical equipment, implants, and stents.
ISSN:2079-4983
2079-4983
DOI:10.3390/jfb13010021