Integrated Metabolomics and Network Pharmacology Investigation of Cardioprotective Effects of Myricetin after 1-Week High-Intensity Exercise

Cardiovascular adverse effects caused by high-intensity exercise (HIE) have become a public health problem of widespread concern. The therapeutic effect and metabolic regulation mechanism of myricetin, a phytochemical with potential therapeutic effects, have rarely been studied. In this study, we es...

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Bibliographic Details
Published in:Nutrients 2023-03, Vol.15 (6), p.1336
Main Authors: Li, Tianyou, Wang, Le, Wu, Luting, Xie, Yingquan, Chang, Mengyun, Wang, Dawei, Yi, Long, Zhu, Xiaohui, Mi, Mantian
Format: Article
Language:English
Subjects:
Animal models
Animals
Antigens
Bioflavonoids
Cardiac function
Cardiovascular diseases
College basketball
Connexin 43
Discriminant analysis
Drinking water
Drug dosages
Drugs, Chinese Herbal - pharmacology
Enzymes
Exercise intensity
Flavones
Flavonoids
Flavonoids - pharmacology
Flavonoids - therapeutic use
Health aspects
Heart
high-intensity exercise
Hypoxia
Injury prevention
Ischemia
Laboratory animals
Medical research
Metabolic networks
Metabolism
Metabolomics
Mice
Molecular docking
Molecular Docking Simulation
molecular mechanism
Myocardium
myricetin
Network Pharmacology
Oxidative stress
Pharmacology
Physiological aspects
Prevention
Public health
Risk factors
Serology
Software
Sports injuries
Therapeutic targets
Transmission electron microscopy
Ultrasonic imaging
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Summary:Cardiovascular adverse effects caused by high-intensity exercise (HIE) have become a public health problem of widespread concern. The therapeutic effect and metabolic regulation mechanism of myricetin, a phytochemical with potential therapeutic effects, have rarely been studied. In this study, we established mice models of different doses of myricetin intervention with 1 week of HIE after intervention. Cardiac function tests, serology, and pathological examinations were used to evaluate the protective effect of myricetin on the myocardium. The possible therapeutic targets of myricetin were obtained using an integrated analysis of metabolomics and network pharmacology and verified using molecular docking and RT-qPCR experiments. Different concentrations of myricetin improved cardiac function, significantly reduced the levels of myocardial injury markers, alleviated myocardial ultrastructural damage, reduced the area of ischemia/hypoxia, and increased the content of CX43. We obtained the potential targets and regulated metabolic network of myricetin by combined network pharmacology and metabolomics analysis and validated them by molecular docking and RT-qPCR. In conclusion, our findings suggest that myricetin exerts anti-cardiac injury effects of HIE through the downregulation of PTGS2 and MAOB and the upregulation of MAP2K1 and EGFR while regulating the complicated myocardial metabolic network.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15061336