Desulfovibrio bacteria enhance alpha-synuclein aggregation in a Caenorhabditis elegans model of Parkinson's disease

The aggregation of the neuronal protein alpha-synuclein (alpha-syn) is a key feature in the pathology of Parkinson's disease (PD). Alpha-syn aggregation has been suggested to be induced in the gut cells by pathogenic gut microbes such as bacteria, which has been shown to be associated with PD....

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Published in:Frontiers in cellular and infection microbiology 2023-05, Vol.13, p.1181315-1181315
Main Authors: Huynh, Vy A, Takala, Timo M, Murros, Kari E, Diwedi, Bidhi, Saris, Per E J
Format: Article
Language:English
Subjects:
alpha-synuclein (alpha-syn)
alpha-Synuclein - genetics
alpha-Synuclein - metabolism
Animals
C. elegans
Caenorhabditis elegans - metabolism
Cellular and Infection Microbiology
Desulfovibrio
Escherichia coli - genetics
Escherichia coli - metabolism
gut Desulfovibrio bacteria
Humans
hydrogen sulfide
lipopolysaccharides
Parkinson Disease
Parkinson’s disease
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Summary:The aggregation of the neuronal protein alpha-synuclein (alpha-syn) is a key feature in the pathology of Parkinson's disease (PD). Alpha-syn aggregation has been suggested to be induced in the gut cells by pathogenic gut microbes such as bacteria, which has been shown to be associated with PD. This study aimed to investigate whether bacteria induce alpha-syn aggregation. Fecal samples of ten PD patients and their healthy spouses were collected for molecular detection of species, followed by bacterial isolation. Isolated strains were used as diets to feed nematodes which overexpress human alpha-syn fused with yellow fluorescence protein. Curli-producing MC4100, which has been shown to facilitate alpha-syn aggregation in animal models, was used as a control bacterial strain, and LSR11, incapable of producing curli, was used as another control strain. The head sections of the worms were imaged using confocal microscopy. We also performed survival assay to determine the effect of bacteria on the survival of the nematodes. Statistical analysis revealed that worms fed bacteria from PD patients harbored significantly more (
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2023.1181315