Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer

To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that i...

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Bibliographic Details
Published in:Animal cells and systems 2020-03, Vol.24 (2), p.107-113
Main Authors: Zhou, Yong, Hu, Xiao-Wei, Yang, Si-Jia, Yu, Zhe
Format: Article
Language:English
Subjects:
Bax protein
Bcl-2 protein
biomarker
Biomarkers
Cell growth
Cell proliferation
Gelatinase A
Gelatinase B
Kinases
LncRNAZFAS1
Lung cancer
Metastases
Metastasis
Neurobiology & Physiology
non-small cell lung cancer (NSCLC)
Non-small cell lung carcinoma
proliferation and metastasis
Proteins
Small cell lung carcinoma
Tissues
Tumors
Xenografts
Xenotransplantation
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Summary:To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissue, especially significantly up-regulated in stage III / IV and in metastatic NSCLC tissues. LncRNAZFAS1 expression was dramatically up-regulated in 4 NSCLC-related cells (A549, SPC-A1, SK-MES-1, and NCI-H1299), with having the highest expression level in A549 cells. Furthermore, we implemented a knockdown of LncRNAZFAS1 in A549 cells, and the results of CCK8 and Transwell assays suggested that knockdown of LncRNAZFAS1 significantly inhibited NSCLC cell proliferation and metastasis. Next, we constructed a tumor xenograft model to evaluate the effect of LncRNAZFAS1 on the NSCLC cell proliferation in vivo. The results indicated that knockdown of LncRNAZFAS1 dramatically inhibited A549 cells proliferation and repressed tumor growth. Additionally, knockdown of LncRNAZFAS1 drastically weakened the expressions of MMP2, MMP9 and Bcl-2 proteins, whereas noticeably strengthened the expression of BAX protein. Our results altogether suggest that knockdown of LncRNAZFAS1 has a negative effect on the proliferation and metastasis of NSCLC cell, which implying LncRNAZFAS1 is a potential unfavorable biomarker in patients with NSCLC.
ISSN:1976-8354
2151-2485
DOI:10.1080/19768354.2020.1736623