Loading…

Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening

The use of natural products (NPs) as possible alternative biocidal compounds for use in antifouling coatings has been the focus of research over the past decades. Despite the importance of this field, the efficacy of a given NP against biofilm (mainly bacteria and diatoms) formation is tested with t...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2018-05, Vol.19 (5), p.1434
Main Authors: Salta, Maria, Dennington, Simon P, Wharton, Julian A
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3
cites cdi_FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3
container_end_page
container_issue 5
container_start_page 1434
container_title International journal of molecular sciences
container_volume 19
creator Salta, Maria
Dennington, Simon P
Wharton, Julian A
description The use of natural products (NPs) as possible alternative biocidal compounds for use in antifouling coatings has been the focus of research over the past decades. Despite the importance of this field, the efficacy of a given NP against biofilm (mainly bacteria and diatoms) formation is tested with the NP being in solution, while almost no studies test the effect of an NP once incorporated into a coating system. The development of a novel bioassay to assess the activity of NP-containing and biocide-containing coatings against marine biofilm formation has been achieved using a high-throughput microplate reader and highly sensitive confocal laser scanning microscopy (CLSM), as well as nucleic acid staining. Juglone, an isolated NP that has previously shown efficacy against bacterial attachment, was incorporated into a simple coating matrix. Biofilm formation over 48 h was assessed and compared against coatings containing the NP and the commonly used booster biocide, cuprous oxide. Leaching of the NP from the coating was quantified at two time points, 24 h and 48 h, showing evidence of both juglone and cuprous oxide being released. Results from the microplate reader showed that the NP coatings exhibited antifouling efficacy, significantly inhibiting biofilm formation when compared to the control coatings, while NP coatings and the cuprous oxide coatings performed equally well. CLSM results and COMSTAT analysis on biofilm 3D morphology showed comparable results when the NP coatings were tested against the controls, with higher biofilm biovolume and maximum thickness being found on the controls. This new method proved to be repeatable and insightful and we believe it is applicable in antifouling and other numerous applications where interactions between biofilm formation and surfaces is of interest.
doi_str_mv 10.3390/ijms19051434
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_30dede5d069d4711a5d39ad8f2546496</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_30dede5d069d4711a5d39ad8f2546496</doaj_id><sourcerecordid>2056452540</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3</originalsourceid><addsrcrecordid>eNpdkktv1DAQgCMEog-4cUaWuPRAqN-JL0iwonSlqkWiPVuO7SReJfZiO5X67-tlS7XlNH5882lmNFX1AcEvhAh47jZzQgIyRAl9VR0jinENIW9eH5yPqpOUNhBigpl4Wx1h0dC2ZBxX03cXejfNYO1H17nsggfdA7gO93YC1yovUU3gVwxm0bkGyhtQErQztl4Fn5Xzzg9gFVQuMYG7tLteumGsb8cYlmHcLhn81tHaHfiuetOrKdn3T_G0urv4cbu6rK9ufq5X365qTZs211SZ0hCiimlEOFbIMMi6zsKetb0hLSIY9UL0VJGONsQq3mHECTOWW9vpjpxW673XBLWR2-hmFR9kUE7-fQhxkCpmpycrCTTWWGYgF4Y2CClmiFCm7TGjnApeXF_3ru3SzdZo63MZyQvpyx_vRjmEe8lES1qIiuDsSRDDn8WmLGeXtJ0m5W1YksSQtJhzxHBBP_2HbsISfRlVoRinrBQFC_V5T-kYUoq2fy4GQblbCXm4EgX_eNjAM_xvB8gjbSGy1A</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2056452540</pqid></control><display><type>article</type><title>Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Salta, Maria ; Dennington, Simon P ; Wharton, Julian A</creator><creatorcontrib>Salta, Maria ; Dennington, Simon P ; Wharton, Julian A</creatorcontrib><description>The use of natural products (NPs) as possible alternative biocidal compounds for use in antifouling coatings has been the focus of research over the past decades. Despite the importance of this field, the efficacy of a given NP against biofilm (mainly bacteria and diatoms) formation is tested with the NP being in solution, while almost no studies test the effect of an NP once incorporated into a coating system. The development of a novel bioassay to assess the activity of NP-containing and biocide-containing coatings against marine biofilm formation has been achieved using a high-throughput microplate reader and highly sensitive confocal laser scanning microscopy (CLSM), as well as nucleic acid staining. Juglone, an isolated NP that has previously shown efficacy against bacterial attachment, was incorporated into a simple coating matrix. Biofilm formation over 48 h was assessed and compared against coatings containing the NP and the commonly used booster biocide, cuprous oxide. Leaching of the NP from the coating was quantified at two time points, 24 h and 48 h, showing evidence of both juglone and cuprous oxide being released. Results from the microplate reader showed that the NP coatings exhibited antifouling efficacy, significantly inhibiting biofilm formation when compared to the control coatings, while NP coatings and the cuprous oxide coatings performed equally well. CLSM results and COMSTAT analysis on biofilm 3D morphology showed comparable results when the NP coatings were tested against the controls, with higher biofilm biovolume and maximum thickness being found on the controls. This new method proved to be repeatable and insightful and we believe it is applicable in antifouling and other numerous applications where interactions between biofilm formation and surfaces is of interest.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms19051434</identifier><identifier>PMID: 29748514</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antifouling coatings ; Bacteria - drug effects ; Bacteria - ultrastructure ; bioassay ; Biocides ; Biofilms ; Biofilms - drug effects ; Biological Products - chemistry ; Coating effects ; Coatings ; Confocal microscopy ; Copper oxides ; Diatoms - drug effects ; Diatoms - ultrastructure ; Disinfectants - chemistry ; High-throughput screening ; High-Throughput Screening Assays ; Juglone ; Leaching ; Microscopy, Confocal ; Natural products ; Nucleic acids ; Oxide coatings ; Protective coatings ; Scanning microscopy</subject><ispartof>International journal of molecular sciences, 2018-05, Vol.19 (5), p.1434</ispartof><rights>Copyright MDPI AG 2018</rights><rights>2018 by the authors. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3</citedby><cites>FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2056452540/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2056452540?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29748514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salta, Maria</creatorcontrib><creatorcontrib>Dennington, Simon P</creatorcontrib><creatorcontrib>Wharton, Julian A</creatorcontrib><title>Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The use of natural products (NPs) as possible alternative biocidal compounds for use in antifouling coatings has been the focus of research over the past decades. Despite the importance of this field, the efficacy of a given NP against biofilm (mainly bacteria and diatoms) formation is tested with the NP being in solution, while almost no studies test the effect of an NP once incorporated into a coating system. The development of a novel bioassay to assess the activity of NP-containing and biocide-containing coatings against marine biofilm formation has been achieved using a high-throughput microplate reader and highly sensitive confocal laser scanning microscopy (CLSM), as well as nucleic acid staining. Juglone, an isolated NP that has previously shown efficacy against bacterial attachment, was incorporated into a simple coating matrix. Biofilm formation over 48 h was assessed and compared against coatings containing the NP and the commonly used booster biocide, cuprous oxide. Leaching of the NP from the coating was quantified at two time points, 24 h and 48 h, showing evidence of both juglone and cuprous oxide being released. Results from the microplate reader showed that the NP coatings exhibited antifouling efficacy, significantly inhibiting biofilm formation when compared to the control coatings, while NP coatings and the cuprous oxide coatings performed equally well. CLSM results and COMSTAT analysis on biofilm 3D morphology showed comparable results when the NP coatings were tested against the controls, with higher biofilm biovolume and maximum thickness being found on the controls. This new method proved to be repeatable and insightful and we believe it is applicable in antifouling and other numerous applications where interactions between biofilm formation and surfaces is of interest.</description><subject>Antifouling coatings</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - ultrastructure</subject><subject>bioassay</subject><subject>Biocides</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biological Products - chemistry</subject><subject>Coating effects</subject><subject>Coatings</subject><subject>Confocal microscopy</subject><subject>Copper oxides</subject><subject>Diatoms - drug effects</subject><subject>Diatoms - ultrastructure</subject><subject>Disinfectants - chemistry</subject><subject>High-throughput screening</subject><subject>High-Throughput Screening Assays</subject><subject>Juglone</subject><subject>Leaching</subject><subject>Microscopy, Confocal</subject><subject>Natural products</subject><subject>Nucleic acids</subject><subject>Oxide coatings</subject><subject>Protective coatings</subject><subject>Scanning microscopy</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkktv1DAQgCMEog-4cUaWuPRAqN-JL0iwonSlqkWiPVuO7SReJfZiO5X67-tlS7XlNH5882lmNFX1AcEvhAh47jZzQgIyRAl9VR0jinENIW9eH5yPqpOUNhBigpl4Wx1h0dC2ZBxX03cXejfNYO1H17nsggfdA7gO93YC1yovUU3gVwxm0bkGyhtQErQztl4Fn5Xzzg9gFVQuMYG7tLteumGsb8cYlmHcLhn81tHaHfiuetOrKdn3T_G0urv4cbu6rK9ufq5X365qTZs211SZ0hCiimlEOFbIMMi6zsKetb0hLSIY9UL0VJGONsQq3mHECTOWW9vpjpxW673XBLWR2-hmFR9kUE7-fQhxkCpmpycrCTTWWGYgF4Y2CClmiFCm7TGjnApeXF_3ru3SzdZo63MZyQvpyx_vRjmEe8lES1qIiuDsSRDDn8WmLGeXtJ0m5W1YksSQtJhzxHBBP_2HbsISfRlVoRinrBQFC_V5T-kYUoq2fy4GQblbCXm4EgX_eNjAM_xvB8gjbSGy1A</recordid><startdate>20180510</startdate><enddate>20180510</enddate><creator>Salta, Maria</creator><creator>Dennington, Simon P</creator><creator>Wharton, Julian A</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20180510</creationdate><title>Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening</title><author>Salta, Maria ; Dennington, Simon P ; Wharton, Julian A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antifouling coatings</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - ultrastructure</topic><topic>bioassay</topic><topic>Biocides</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biological Products - chemistry</topic><topic>Coating effects</topic><topic>Coatings</topic><topic>Confocal microscopy</topic><topic>Copper oxides</topic><topic>Diatoms - drug effects</topic><topic>Diatoms - ultrastructure</topic><topic>Disinfectants - chemistry</topic><topic>High-throughput screening</topic><topic>High-Throughput Screening Assays</topic><topic>Juglone</topic><topic>Leaching</topic><topic>Microscopy, Confocal</topic><topic>Natural products</topic><topic>Nucleic acids</topic><topic>Oxide coatings</topic><topic>Protective coatings</topic><topic>Scanning microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salta, Maria</creatorcontrib><creatorcontrib>Dennington, Simon P</creatorcontrib><creatorcontrib>Wharton, Julian A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salta, Maria</au><au>Dennington, Simon P</au><au>Wharton, Julian A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2018-05-10</date><risdate>2018</risdate><volume>19</volume><issue>5</issue><spage>1434</spage><pages>1434-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The use of natural products (NPs) as possible alternative biocidal compounds for use in antifouling coatings has been the focus of research over the past decades. Despite the importance of this field, the efficacy of a given NP against biofilm (mainly bacteria and diatoms) formation is tested with the NP being in solution, while almost no studies test the effect of an NP once incorporated into a coating system. The development of a novel bioassay to assess the activity of NP-containing and biocide-containing coatings against marine biofilm formation has been achieved using a high-throughput microplate reader and highly sensitive confocal laser scanning microscopy (CLSM), as well as nucleic acid staining. Juglone, an isolated NP that has previously shown efficacy against bacterial attachment, was incorporated into a simple coating matrix. Biofilm formation over 48 h was assessed and compared against coatings containing the NP and the commonly used booster biocide, cuprous oxide. Leaching of the NP from the coating was quantified at two time points, 24 h and 48 h, showing evidence of both juglone and cuprous oxide being released. Results from the microplate reader showed that the NP coatings exhibited antifouling efficacy, significantly inhibiting biofilm formation when compared to the control coatings, while NP coatings and the cuprous oxide coatings performed equally well. CLSM results and COMSTAT analysis on biofilm 3D morphology showed comparable results when the NP coatings were tested against the controls, with higher biofilm biovolume and maximum thickness being found on the controls. This new method proved to be repeatable and insightful and we believe it is applicable in antifouling and other numerous applications where interactions between biofilm formation and surfaces is of interest.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>29748514</pmid><doi>10.3390/ijms19051434</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1422-0067
ispartof International journal of molecular sciences, 2018-05, Vol.19 (5), p.1434
issn 1422-0067
1661-6596
1422-0067
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_30dede5d069d4711a5d39ad8f2546496
source Publicly Available Content Database; PubMed Central
subjects Antifouling coatings
Bacteria - drug effects
Bacteria - ultrastructure
bioassay
Biocides
Biofilms
Biofilms - drug effects
Biological Products - chemistry
Coating effects
Coatings
Confocal microscopy
Copper oxides
Diatoms - drug effects
Diatoms - ultrastructure
Disinfectants - chemistry
High-throughput screening
High-Throughput Screening Assays
Juglone
Leaching
Microscopy, Confocal
Natural products
Nucleic acids
Oxide coatings
Protective coatings
Scanning microscopy
title Biofilm Inhibition by Novel Natural Product- and Biocide-Containing Coatings Using High-Throughput Screening
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A46%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biofilm%20Inhibition%20by%20Novel%20Natural%20Product-%20and%20Biocide-Containing%20Coatings%20Using%20High-Throughput%20Screening&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Salta,%20Maria&rft.date=2018-05-10&rft.volume=19&rft.issue=5&rft.spage=1434&rft.pages=1434-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms19051434&rft_dat=%3Cproquest_doaj_%3E2056452540%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c478t-4ad19014a5c1362a1d505bbe0f58fd381321f99f4a3b473ea6b21635de6eebcb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2056452540&rft_id=info:pmid/29748514&rfr_iscdi=true