Comprehensive and quantitative analysis of intracellular structure polarization at the apical–basal axis in elongating Arabidopsis zygotes

A comprehensive and quantitative evaluation of multiple intracellular structures or proteins is a promising approach to provide a deeper understanding of and new insights into cellular polarity. In this study, we developed an image analysis pipeline to obtain intensity profiles of fluorescent probes...

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Bibliographic Details
Published in:Scientific reports 2023-12, Vol.13 (1), p.22879-11, Article 22879
Main Authors: Hiromoto, Yukiko, Minamino, Naoki, Kikuchi, Suzuka, Kimata, Yusuke, Matsumoto, Hikari, Nakagawa, Sakumi, Ueda, Minako, Higaki, Takumi
Format: Article
Language:English
Subjects:
631/114
631/449
631/80
Actin
Cell division
Elongation
Fertilization
Filaments
Fluorescent indicators
Humanities and Social Sciences
Image processing
Intracellular
Microtubules
multidisciplinary
Science
Science (multidisciplinary)
Zygotes
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Summary:A comprehensive and quantitative evaluation of multiple intracellular structures or proteins is a promising approach to provide a deeper understanding of and new insights into cellular polarity. In this study, we developed an image analysis pipeline to obtain intensity profiles of fluorescent probes along the apical–basal axis in elongating Arabidopsis thaliana zygotes based on two-photon live-cell imaging data. This technique showed the intracellular distribution of actin filaments, mitochondria, microtubules, and vacuolar membranes along the apical–basal axis in elongating zygotes from the onset of cell elongation to just before asymmetric cell division. Hierarchical cluster analysis of the quantitative data on intracellular distribution revealed that the zygote may be compartmentalized into two parts, with a boundary located 43.6% from the cell tip, immediately after fertilization. To explore the biological significance of this compartmentalization, we examined the positions of the asymmetric cell divisions from the dataset used in this distribution analysis. We found that the cell division plane was reproducibly inserted 20.5% from the cell tip. This position corresponded well with the midpoint of the compartmentalized apical region, suggesting a potential relationship between the zygote compartmentalization, which begins with cell elongation, and the position of the asymmetric cell division.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-50020-8