Highly Sensitive and Selective Detection of L-Tryptophan by ECL Using Boron-Doped Diamond Electrodes

L-tryptophan is an amino acid that is essential to the metabolism of humans. Therefore, there is a high interest for its detection in biological fluids including blood, urine, and saliva for medical studies, but also in food products. Towards this goal, we report on a new electrochemiluminescence (E...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2024-06, Vol.24 (11), p.3627
Main Authors: Scorsone, Emmanuel, Stewart, Samuel, Hamel, Matthieu
Format: Article
Language:English
Subjects:
Amino acids
Aqueous solutions
Biosensing Techniques - methods
Boron
Boron - chemistry
boron-doped diamond
Carbon
Chemical vapor deposition
Detectors
Diamond - chemistry
Electrochemical Techniques - methods
electrochemiluminescence
Electrodes
Electrons
High performance liquid chromatography
Humans
Hydrogen peroxide
Hydrogen Peroxide - analysis
Hydrogen Peroxide - chemistry
L-tryptophan
Limit of Detection
Luminescent Measurements - methods
Methods
Oxidation
Phosphates
Silicon wafers
Stereoisomers
Tryptophan
Tryptophan - analysis
Tryptophan - chemistry
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Summary:L-tryptophan is an amino acid that is essential to the metabolism of humans. Therefore, there is a high interest for its detection in biological fluids including blood, urine, and saliva for medical studies, but also in food products. Towards this goal, we report on a new electrochemiluminescence (ECL) method for L-tryptophan detection involving the in situ production of hydrogen peroxide at the surface of boron-doped diamond (BDD) electrodes. We demonstrate that the ECL response efficiency is directly related to H O production at the electrode surface and propose a mechanism for the ECL emission of L-tryptophan. After optimizing the analytical conditions, we show that the ECL response to L-tryptophan is directly linear with concentration in the range of 0.005 to 1 µM. We achieved a limit of detection of 0.4 nM and limit of quantification of 1.4 nM in phosphate buffer saline (PBS, pH 7.4). Good selectivity against other indolic compounds (serotonin, 3-methylindole, tryptamine, indole) potentially found in biological fluids was observed, thus making this approach highly promising for quantifying L-tryptophan in a broad range of aqueous matrices of interest.
ISSN:1424-8220
1424-8220
DOI:10.3390/s24113627