A green approach for metoclopramide quantification in pharmaceutical products: new HPLC and spectrophotometric methods

Green spectrophotometric and HPLC methods have been developed for the quantification of metoclopramide. In the spectrophotometric method, it was determined by direct absorbance measurement at 273 nm wavelength using ultrapure water as solvent. The Extend C18 column was used for the HPLC method. The...

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Bibliographic Details
Published in:Scientific reports 2024-04, Vol.14 (1), p.8765-8765, Article 8765
Main Authors: Demir, İbrahim, Bulduk, İbrahim, Darwısh, Ibrahim A., Enginar, Hüseyin
Format: Article
Language:English
Subjects:
704/172
704/172/169
704/172/4081
AGREE
Chromatography, High Pressure Liquid - methods
Ethanol
Flow rates
Formic acid
Green HPLC
Green spectrophotometry
High-performance liquid chromatography
Humanities and Social Sciences
Liquid chromatography
Metoclopramide
Metoclopramide - analysis
multidisciplinary
Pharmaceutical Preparations
Pharmaceuticals
Science
Science (multidisciplinary)
Solvents
Spectrophotometry
Water
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Summary:Green spectrophotometric and HPLC methods have been developed for the quantification of metoclopramide. In the spectrophotometric method, it was determined by direct absorbance measurement at 273 nm wavelength using ultrapure water as solvent. The Extend C18 column was used for the HPLC method. The mobile phase system consisted of a combination of ethanol and formic acid solution (pH 2.0; 30:70 v/v). Isocratic elution was applied and the flow rate was set at 1.0 mL min −1 . Metoclopramide was detected at 273 nm. The methods performed were economical, rapid, environmentally friendly, and simple, providing metoclopramide analysis within 5 min. The methods have been successfully applied in pharmaceutical products without matrix interference. The results of the application of the developed methods to pharmaceutical products were statistically compared and no significant difference was observed between the methods. In addition, the greenness assessment of the developed methods was performed using AGREE software. Our developed methods, based on the use of solvents such as ethanol and water, are proposed as a more environmentally and analyst-friendly option for the quantification of metoclopramide in pharmaceutical products than other methods currently in use.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-59149-6