Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis

Aims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We inves...

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Bibliographic Details
Published in:Gastroenterology research and practice 2017-01, Vol.2017 (2017), p.1-9
Main Authors: McClain, Craig J., Luan, Weimin, Feng, Wenke, Yin, Baishuang, Zhuang, Xinyu, Ren, Li, Shao, Tuo, Kong, Xiaoxia, Yang, Ying, Zhao, Cuiqing, Liu, Liming, Dryden, Gerald
Format: Article
Language:English
Subjects:
Adipocytes
Alcohol
Animal sciences
Body fat
Colitis
Development and progression
Drug dosages
Enzymes
Fatty acids
Fibroblast growth factors
Fibroblasts
Growth factors
Health aspects
Inflammatory bowel disease
Interleukin-6
Kinases
Lipids
Lipolysis
Metabolism
Metabolites
Phosphorylation
Physiological aspects
Proteins
Rodents
Studies
Veterinary medicine
Zoology
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Summary:Aims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We investigated the impact of FGF21 in experimental colitis-induced epididymal white adipose tissue (eWAT) lipolysis. Methods. Mice were given 2.5% dextran sulfate sodium (DSS) ad libitum for 7 days to induce colitis. The role of FGF21 was investigated using antibody neutralization or knockout (KO) mice. Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. Results. DSS markedly decreased eWAT/body weight ratio and increased serum concentrations of free fatty acid (FFA) and glycerol, indicating increased adipose tissue lipolysis. eWAT intracellular lipolytic enzyme expression/activation was significantly increased. These alterations were significantly attenuated in FGF21 KO mice and by circulating FGF21 neutralization. Moreover, DSS treatment markedly increased serum IL-6 and FGF21 levels. IL-6 pretreatment was necessary for the stimulatory effect of FGF21 on adipose lipolysis in 3T3-L1 cells. Conclusions. Our results demonstrate that experimental colitis induces eWAT lipolysis via an IL-6/FGF21-mediated signaling pathway.
ISSN:1687-6121
1687-630X
DOI:10.1155/2017/3089378