Tracking Permeation of Dimethyl Sulfoxide (DMSO) in Mentha × piperita Shoot Tips Using Coherent Raman Microscopy

Cryopreservation has emerged as a low-maintenance, cost-effective solution for the long-term preservation of vegetatively propagated crops. Shoot tip cryopreservation often makes use of vitrification methods that employ highly concentrated mixtures of cryoprotecting agents; however, little is unders...

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Bibliographic Details
Published in:Plants (Basel) 2023-06, Vol.12 (12), p.2247-2247
Main Authors: Kreckel, Heidi D, Samuels, Fionna M D, Bonnart, Remi, Volk, Gayle M, Stich, Dominik G, Levinger, Nancy E
Format: Article
Language:English
Subjects:
brightfield microscopy
CARS microscopy
Coherent scattering
cost effectiveness
Cryopreservation
cryoprotectant distribution
Dimethyl sulfoxide
Freezing
Glycerol
Leaves
Localization
Mentha
Mentha piperita
Microscopy
peppermint
permeability
plant cryopreservation
Raman
Raman spectra
Shoots
Spectrum analysis
Sulfoxides
vegetative propagation
Vitrification
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Summary:Cryopreservation has emerged as a low-maintenance, cost-effective solution for the long-term preservation of vegetatively propagated crops. Shoot tip cryopreservation often makes use of vitrification methods that employ highly concentrated mixtures of cryoprotecting agents; however, little is understood as to how these cryoprotecting agents protect cells and tissues from freezing. In this study, we use coherent anti-Stokes Raman scattering microscopy to directly visualize where dimethyl sulfoxide (DMSO) localizes within shoot tips. We find that DMSO fully penetrates the shoot tip tissue within 10 min of exposure. Variations in signal intensities across images suggest that DMSO may interact with cellular components, leading to its accumulation in specific regions.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12122247