Determination of Epimedin B in Rat Plasma and Tissue by LC-MS/MS: Application in Pharmacokinetic and Tissue Distribution Studies

A simple, sensitive, and specific liquid chromatography tandem mass-spectrometric method was developed and validated for the determination of epimedin B in rat plasma and tissue samples. After being processed with a protein precipitation method, these samples were separated on an Agilent Eclipse XDB...

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Published in:Journal of analytical methods in chemistry 2017-01, Vol.2017 (2017), p.1-9
Main Authors: Sun, Shuai, Yu, Jiejing, Xu, Shunjun, Feng, Qianru, Yang, Liu
Format: Article
Language:English
Subjects:
Bioavailability
Biological products
Flavonoids
Liquid chromatography
Medical research
Medicine, Experimental
Methods
Mice
Osteoporosis
Pharmaceuticals
Pharmacokinetics
Physiological aspects
Plasma
Reproductive system
Rodents
Studies
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creator Sun, Shuai
Yu, Jiejing
Xu, Shunjun
Feng, Qianru
Yang, Liu
description A simple, sensitive, and specific liquid chromatography tandem mass-spectrometric method was developed and validated for the determination of epimedin B in rat plasma and tissue samples. After being processed with a protein precipitation method, these samples were separated on an Agilent Eclipse XDB-C18 column with an isocratic mobile phase consisting of acetonitrile and 0.1% formic acid aqueous solution (32 : 68, v/v). The calibration curve of epimedin B was linear over the concentration range from 1 to 500 ng/mL in plasma and tissue homogenate. The method was then applied to pharmacokinetic and tissue distribution studies after a single oral administration of Herba Epimedii extract to SD rats. Results showed that epimedin B reached the plasma peak concentration at 0.4 h and the terminal elimination half-life was 1.6 h in rat plasma, and the plasma area under the curve from time zero to infinity (AUC0–∞) was 14.35 μg/L·h. The concentration distribution of epimedin B in rat tissue was in the following order: liver > ovary > womb > lung > kidney > spleen > heart > brain, indicating that the compound could be widely distributed in rat, and the reproductive system may be the principal target of epimedin B for female rat.
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subjects Bioavailability
Biological products
Flavonoids
Liquid chromatography
Medical research
Medicine, Experimental
Methods
Mice
Osteoporosis
Pharmaceuticals
Pharmacokinetics
Physiological aspects
Plasma
Reproductive system
Rodents
Studies
title Determination of Epimedin B in Rat Plasma and Tissue by LC-MS/MS: Application in Pharmacokinetic and Tissue Distribution Studies
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