MPP8 Governs the Activity of the LIF/STAT3 Pathway and Plays a Crucial Role in the Differentiation of Mouse Embryonic Stem Cells

Mouse embryonic stem cells (mESCs) possess the remarkable characteristics of unlimited self-renewal and pluripotency, which render them highly valuable for both fundamental research and clinical applications. A comprehensive understanding of the molecular mechanisms underlying mESC function is of th...

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Published in:Cells (Basel, Switzerland) Switzerland), 2023-08, Vol.12 (16), p.2023
Main Authors: Zhang, Heyao, Yang, Tenghui, Wu, Hao, Yi, Wen, Dai, Chunhong, Chen, Xi, Zhang, Wensheng, Ye, Ying
Format: Article
Language:English
Subjects:
Cell culture
Cell cycle
Cell differentiation
Cell lines
Cell self-renewal
Cloning
Data analysis
differentiation
DNA methylation
Embryo cells
Embryogenesis
Epigenetics
Experiments
Genes
LIF/STAT3 signaling pathway
mESCs
Molecular modelling
MPP8
Nanog
Phosphatase
Pluripotency
Proteins
Signal transduction
Stat3 protein
Statistical analysis
Stem cells
Transposons
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Summary:Mouse embryonic stem cells (mESCs) possess the remarkable characteristics of unlimited self-renewal and pluripotency, which render them highly valuable for both fundamental research and clinical applications. A comprehensive understanding of the molecular mechanisms underlying mESC function is of the utmost importance. The Human Silence Hub (HUSH) complex, comprising FAM208A, MPP8, and periphilin, constitutes an epigenetic silencing complex involved in suppressing retroviruses and transposons during early embryonic development. However, its precise role in regulating mESC pluripotency and differentiation remains elusive. In this study, we generated homogenous miniIAA7-tagged Mpp8 mouse ES cell lines. Upon induction of MPP8 protein degradation, we observed the impaired proliferation and reduced colony formation ability of mESCs. Furthermore, this study unveils the involvement of MPP8 in regulating the activity of the LIF/STAT3 signaling pathway and Nanog expression in mESCs. Finally, we provide compelling evidence that degradation of the MPP8 protein impairs the differentiation of mESC.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells12162023