Genome-wide characterization of NtHD-ZIP IV: different roles in abiotic stress response and glandular Trichome induction

• Published in: BMC plant biology 2019-10, Vol.19 (1), p.444-444, Article 444
• Main Authors: Zhang, Hongying, Ma, Xudong, Li, Wenjiao, Niu, Dexin, Wang, Zhaojun, Yan, Xiaoxiao, Yang, Xinling, Yang, Yongfeng, Cui, Hong
• Format: Article
• Language: English
• Subjects: ABA; Abscisic acid; Amino acids; Analysis; Cytokinins; DNA binding proteins; Expression pattern; Gene Expression Regulation, Plant - genetics; Gene Regulatory Networks; Genes; Genetic aspects; Genetic research; Genomes; Genomics; Gibberellins; Growth; High definition television; Leucine Zippers; MeJA; Nicotiana; Nicotiana - genetics; Nicotiana - physiology; Nicotiana tabacum; Phenols (Class of compounds); Plant genetics; Plant hormones; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Stress, Physiological; Trichomes - genetics; Trichomes - physiology
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-019-2023-4

The plant-specific homeodomain-leucine zipper class IV (HD-ZIP IV) gene family has been involved in the regulation of epidermal development. Fifteen genes coding for HD-ZIP IV proteins were identified (NtHD-ZIP-IV-1 to NtHD-ZIP-IV-15) based on the genome of N. tabacum. Four major domains (HD, ZIP, SAD and START) were present in these proteins. Tissue expression pattern analysis indicated that NtHD-ZIP-IV-1, - 2, - 3, - 10, and - 12 may be associated with trichome development; NtHD-ZIP-IV-8 was expressed only in cotyledons; NtHD-ZIP-IV-9 only in the leaf and stem epidermis; NtHD-ZIP-IV-11 only in leaves; and NtHD-ZIP-IV-15 only in the root and stem epidermis. We found that jasmonates may induce the generation of glandular trichomes, and that NtHD-ZIP-IV-1, - 2, - 5, and - 7 were response to MeJA treatment. Dynamic expression under abiotic stress and after application of phytohormones indicated that most NtHD-ZIP IV genes were induced by heat, cold, salt and drought. Furthermore, most of these genes were induced by gibberellic acid, 6-benzylaminopurine, and salicylic acid, but were inhibited by abscisic acid. NtHD-ZIP IV genes were sensitive to heat, but insensitive to osmotic stress. NtHD-ZIP IV genes are implicated in a complex regulatory gene network controlling epidermal development and abiotic stress responses. The present study provides evidence to elucidate the gene functions of NtHD-ZIP IVs during epidermal development and stress response.