Photoacoustic lifetime oxygen imaging of radiotherapy-induced tumor reoxygenation In Vivo

• Published in: Journal of photochemistry and photobiology 2024-06, Vol.21, p.100241, Article 100241
• Main Authors: Folz, Jeff, Jo, Janggun, Gonzalez, Maria E., Eido, Ahmad, Zhai, Tianqu, Caruso, Roberta, Kleer, Celina G., Wang, Xueding, Kopelman, Raoul
• Format: Article
• Language: English
• Subjects: Cancer; Nanosensors; PACI; Photoacoustics; Radiation; Reoxygenation
• ISSN: 2666-4690; 2666-4690
• DOI: 10.1016/j.jpap.2024.100241

Early detection and diagnosis of cancer is critical for achieving positive therapeutic outcomes. Biomarkers that can provide clinicians with clues to the outcome of a given therapeutic course are highly desired. Oxygen is a small molecule that is nearly universally present in biological tissues and plays a critical role in the effectiveness of radiotherapies by reacting with DNA radicals and subsequently impairing cellular repair of double strand breaks. Techniques for measuring oxygen in biological tissues often use blood oxygen saturation to approximate the oxygen partial pressure in surrounding tissues despite the complex, nonlinear, and dynamic relationship between these two separate oxygen populations. We combined a directly oxygen-sensitive, tumor-targeted, chemical contrast nanoelement with the photoacoustic lifetime-based (PALT) oxygen imaging technique to obtain image maps of oxygen in breast cancer tumors in vivo. The oxygen levels of patient-derived xenografts in a mouse model were characterized before and after a course of radiotherapy. We show that, independent of tumor size, radiotherapy induced an increase in the overall oxygenation levels of the tumor. Further, this increase in the oxygenation of the tumor significantly correlated with a positive response to radiotherapy, as demonstrated by a reduction in tumor volume over the twenty-day monitoring period following therapy and histological staining. Our PALT imaging presented here is simple, fast, and non-invasive. Facilized by the PALT approach, imaging of tumor reoxygenation may be utilized as a simple, early indicator for evaluating cancer response to radiotherapy. Further characterization of the reoxygenation degree, temporal onset, and possible theragnostic implications are warranted.