Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model

The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenog...

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Bibliographic Details
Published in:STAR protocols 2024-06, Vol.5 (2), p.102968, Article 102968
Main Authors: Colenbier, Robin, Coppens, Lenny, Logghe, Tine, van Zwol, Eke, Timmermans, Jean-Pierre, Bogers, Johannes
Format: Article
Language:English
Subjects:
Cancer
Cell culture
Model Organisms
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Summary:The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality. [Display omitted] •Steps for embryo incubation and preparation•Precautions to ensure optimal embryo viability and experimental success•Generation of single-cell grafting suspension for tumor induction•Instructions for CAM abrasion Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2024.102968