Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica

We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane cont...

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Bibliographic Details
Published in:Energies (Basel) 2013-04, Vol.6 (4), p.2052-2064
Main Authors: Kuan, I-Ching, Lee, Chia-Chi, Tsai, Bing-Hong, Lee, Shiow-Ling, Lee, Wei-Ting, Yu, Chi-Yang
Format: Article
Language:English
Subjects:
biodiesel
Biodiesel fuels
biomimetic silica
Cellulose acetate
Cooking
Design of experiments
Enzymes
Glycerol
lipase
Nanoparticles
Raw materials
response surface methodology
Soybeans
Vegetable oils
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Summary:We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.
ISSN:1996-1073
1996-1073
DOI:10.3390/en6042052