Prognostic Relevance of CD4+, CD8+ and FOXP3+ TILs in Oral Squamous Cell Carcinoma and Correlations with PD-L1 and Cancer Stem Cell Markers

This study investigates the relevance of tumor-infiltrating lymphocytes (TILs) in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis of stromal/tumoral CD4+, CD8+ and FOXP3+ TILs is performed in 125 OSCC patients. Potential relationships with the expression of tumoral PD-L1 and cancer...

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Bibliographic Details
Published in:Biomedicines 2021-06, Vol.9 (6), p.653
Main Authors: Lequerica-Fernández, Paloma, Suárez-Canto, Julián, Rodriguez-Santamarta, Tania, Rodrigo, Juan Pablo, Suárez-Sánchez, Faustino Julián, Blanco-Lorenzo, Verónica, Domínguez-Iglesias, Francisco, García-Pedrero, Juana María, de Vicente, Juan Carlos
Format: Article
Language:English
Subjects:
Biomarkers
Cancer therapies
CD4
CD4 antigen
CD8
CD8 antigen
Cell cycle
Cloning
Cytotoxicity
FOXP3
Foxp3 protein
Infiltration
Lymphocytes
Medical prognosis
Metastases
Monoclonal antibodies
Multivariate analysis
Nestin
Oct-4 protein
OCT4
Oral squamous cell carcinoma
Patients
PD-L1 protein
Podoplanin
Radiation therapy
Squamous cell carcinoma
Stem cells
Survival analysis
Tumor-infiltrating lymphocytes
Tumors
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Summary:This study investigates the relevance of tumor-infiltrating lymphocytes (TILs) in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis of stromal/tumoral CD4+, CD8+ and FOXP3+ TILs is performed in 125 OSCC patients. Potential relationships with the expression of tumoral PD-L1 and cancer stem cell (CSC) markers (NANOG, SOX2, OCT4, Nestin and Podoplanin (PDPN)) are assessed. CD4+ and CD8+ TILs are significantly associated with smoking and alcohol habits. CD4+ and CD8+ TILs show an inverse relationship with NANOG and SOX2 expression, and FOXP3+ TILs is significantly correlated with Nestin and PDPN expression. High infiltration of CD4+ and CD8+ TILs and a high tumoral CD8+/FOXP3+ ratio are significantly associated with tumors harboring positive PD-L1 expression. Infiltration of stromal/tumoral FOXP3+ TILs and a low stromal CD8+/FOXP3+ ratio are significantly associated with better disease-specific survival. Multivariate analysis reveals that the stromal CD8+/FOXP3+ TILs ratio is a significant independent prognostic factor. Regarding OSCC patient survival, the CD8+/FOXP3+ TILs ratio is an independent prognostic factor. TILs may act as biomarkers and potential therapeutic targets for OSCC.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9060653