Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker

This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or d...

Full description

Saved in:
Bibliographic Details
Published in:Wārasān Songkhlā Nakharin 2022-08, Vol.44 (4), p.1040-1047
Main Authors: Phochit Nanthanawat, Samnao Saowakoon, Witchuda Prasatkaew, Amnuay Wattanakornsiri, Jakkaphun Nanuam, Chayapol Meeprom, Chutima Thanomsit
Format: Article
Language:English
Subjects:
elisa technique
mortality rate
toxicity testing
western blot
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1047
container_issue 4
container_start_page 1040
container_title Wārasān Songkhlā Nakharin
container_volume 44
creator Phochit Nanthanawat
Samnao Saowakoon
Witchuda Prasatkaew
Amnuay Wattanakornsiri
Jakkaphun Nanuam
Chayapol Meeprom
Chutima Thanomsit
description This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control. The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h was approximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and their eggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISA technique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p
doi_str_mv 10.14456/sjst-psu.2022.136
format article
fullrecord <record><control><sourceid>doaj</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_31e9d590e7e44802ad6fe27ada93961b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_31e9d590e7e44802ad6fe27ada93961b</doaj_id><sourcerecordid>oai_doaj_org_article_31e9d590e7e44802ad6fe27ada93961b</sourcerecordid><originalsourceid>FETCH-LOGICAL-d136t-ed44498656cf61ff179ccc2011b85ea7029fa093ee7318a6645ca7bbffc2db1f3</originalsourceid><addsrcrecordid>eNotj8tOwzAURLMAiVL4AVZeFokUv-LUy6oqUKkSLGAd3djXrYtJIttF6qfwt4THajQjzRlNUdwwOmdSVuo-HVIuh3Scc8r5nAl1Vkwo41UphK4uisuUDpTKMWCT4mtpPzEmJOgcmpxI74g5DRg_MO-j70jfkV0fLHYEhiEgSR34kMjspf8Ag0AMdBC8OQbIcEugsyTv0UeCu126-_U_PW8g-xE10pcG8ymYfR98hyljhHF9tlzt12M9kdaP4PiO8ao4dxASXv_rtHh7WL-unsrt8-NmtdyWdnyWS7RSSr1QlTJOMedYrY0xnDLWLiqEmnLtgGqBWAu2AKVkZaBuW-cMty1zYlps_ri2h0MzRD_On5oefPMb9HHXQMzeBGwEQ20rTbFGKReUg1UOeQ0WtNCKteIbMOF5Aw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker</title><source>EZB Electronic Journals Library</source><creator>Phochit Nanthanawat ; Samnao Saowakoon ; Witchuda Prasatkaew ; Amnuay Wattanakornsiri ; Jakkaphun Nanuam ; Chayapol Meeprom ; Chutima Thanomsit</creator><creatorcontrib>Phochit Nanthanawat ; Samnao Saowakoon ; Witchuda Prasatkaew ; Amnuay Wattanakornsiri ; Jakkaphun Nanuam ; Chayapol Meeprom ; Chutima Thanomsit</creatorcontrib><description>This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control. The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h was approximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and their eggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISA technique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p &lt;0.05). Based on our results, AChE could be applied to assess cypermethrin exposure in the snails and their eggs, in order to plan contamination management of such pesticides in the snails and reduce the risks to consumers.</description><identifier>ISSN: 0125-3395</identifier><identifier>DOI: 10.14456/sjst-psu.2022.136</identifier><language>eng</language><publisher>Prince of Songkla University</publisher><subject>elisa technique ; mortality rate ; toxicity testing ; western blot</subject><ispartof>Wārasān Songkhlā Nakharin, 2022-08, Vol.44 (4), p.1040-1047</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Phochit Nanthanawat</creatorcontrib><creatorcontrib>Samnao Saowakoon</creatorcontrib><creatorcontrib>Witchuda Prasatkaew</creatorcontrib><creatorcontrib>Amnuay Wattanakornsiri</creatorcontrib><creatorcontrib>Jakkaphun Nanuam</creatorcontrib><creatorcontrib>Chayapol Meeprom</creatorcontrib><creatorcontrib>Chutima Thanomsit</creatorcontrib><title>Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker</title><title>Wārasān Songkhlā Nakharin</title><description>This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control. The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h was approximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and their eggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISA technique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p &lt;0.05). Based on our results, AChE could be applied to assess cypermethrin exposure in the snails and their eggs, in order to plan contamination management of such pesticides in the snails and reduce the risks to consumers.</description><subject>elisa technique</subject><subject>mortality rate</subject><subject>toxicity testing</subject><subject>western blot</subject><issn>0125-3395</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNotj8tOwzAURLMAiVL4AVZeFokUv-LUy6oqUKkSLGAd3djXrYtJIttF6qfwt4THajQjzRlNUdwwOmdSVuo-HVIuh3Scc8r5nAl1Vkwo41UphK4uisuUDpTKMWCT4mtpPzEmJOgcmpxI74g5DRg_MO-j70jfkV0fLHYEhiEgSR34kMjspf8Ag0AMdBC8OQbIcEugsyTv0UeCu126-_U_PW8g-xE10pcG8ymYfR98hyljhHF9tlzt12M9kdaP4PiO8ao4dxASXv_rtHh7WL-unsrt8-NmtdyWdnyWS7RSSr1QlTJOMedYrY0xnDLWLiqEmnLtgGqBWAu2AKVkZaBuW-cMty1zYlps_ri2h0MzRD_On5oefPMb9HHXQMzeBGwEQ20rTbFGKReUg1UOeQ0WtNCKteIbMOF5Aw</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Phochit Nanthanawat</creator><creator>Samnao Saowakoon</creator><creator>Witchuda Prasatkaew</creator><creator>Amnuay Wattanakornsiri</creator><creator>Jakkaphun Nanuam</creator><creator>Chayapol Meeprom</creator><creator>Chutima Thanomsit</creator><general>Prince of Songkla University</general><scope>DOA</scope></search><sort><creationdate>20220801</creationdate><title>Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker</title><author>Phochit Nanthanawat ; Samnao Saowakoon ; Witchuda Prasatkaew ; Amnuay Wattanakornsiri ; Jakkaphun Nanuam ; Chayapol Meeprom ; Chutima Thanomsit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d136t-ed44498656cf61ff179ccc2011b85ea7029fa093ee7318a6645ca7bbffc2db1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>elisa technique</topic><topic>mortality rate</topic><topic>toxicity testing</topic><topic>western blot</topic><toplevel>online_resources</toplevel><creatorcontrib>Phochit Nanthanawat</creatorcontrib><creatorcontrib>Samnao Saowakoon</creatorcontrib><creatorcontrib>Witchuda Prasatkaew</creatorcontrib><creatorcontrib>Amnuay Wattanakornsiri</creatorcontrib><creatorcontrib>Jakkaphun Nanuam</creatorcontrib><creatorcontrib>Chayapol Meeprom</creatorcontrib><creatorcontrib>Chutima Thanomsit</creatorcontrib><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Wārasān Songkhlā Nakharin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phochit Nanthanawat</au><au>Samnao Saowakoon</au><au>Witchuda Prasatkaew</au><au>Amnuay Wattanakornsiri</au><au>Jakkaphun Nanuam</au><au>Chayapol Meeprom</au><au>Chutima Thanomsit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker</atitle><jtitle>Wārasān Songkhlā Nakharin</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>44</volume><issue>4</issue><spage>1040</spage><epage>1047</epage><pages>1040-1047</pages><issn>0125-3395</issn><abstract>This study aimed to evaluate the effects of cypermethrin on hatching rate of golden apple snail eggs, mortality rate of the golden apple snails, and acetylcholinesterase (AChE) expression applied as a bio-indicator. The results showed that cypermethrin concentration did not affect hatching rate or development of the eggs and larvae in comparison with the control. The mortality rate depended on the exposure concentration. Median lethal concentration (LC50) (95% confidence) at 96 h was approximately 8.99 (8.93-9.06). The concentration of cypermethrin had an effect on AChE expression in both the snails and their eggs. The molecular weight of AChE found was 71 kDa, as studied by SDS-PAGE and Western blot techniques. The ELISA technique revealed that AChE contents in both the snails and their eggs were significantly different from the control (p &lt;0.05). Based on our results, AChE could be applied to assess cypermethrin exposure in the snails and their eggs, in order to plan contamination management of such pesticides in the snails and reduce the risks to consumers.</abstract><pub>Prince of Songkla University</pub><doi>10.14456/sjst-psu.2022.136</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0125-3395
ispartof Wārasān Songkhlā Nakharin, 2022-08, Vol.44 (4), p.1040-1047
issn 0125-3395
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_31e9d590e7e44802ad6fe27ada93961b
source EZB Electronic Journals Library
subjects elisa technique
mortality rate
toxicity testing
western blot
title Adverse effects of cypermethrin on golden apple snails (Pomacea canaliculata) and their eggs, and application of Acetylcholinesterase (AChE) as biomarker
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-23T22%3A23%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adverse%20effects%20of%20cypermethrin%20on%20golden%20apple%20snails%20(Pomacea%20canaliculata)%20and%20their%20eggs,%20and%20application%20of%20Acetylcholinesterase%20(AChE)%20as%20biomarker&rft.jtitle=W%C4%81ras%C4%81n%20Songkhl%C4%81%20Nakharin&rft.au=Phochit%20Nanthanawat&rft.date=2022-08-01&rft.volume=44&rft.issue=4&rft.spage=1040&rft.epage=1047&rft.pages=1040-1047&rft.issn=0125-3395&rft_id=info:doi/10.14456/sjst-psu.2022.136&rft_dat=%3Cdoaj%3Eoai_doaj_org_article_31e9d590e7e44802ad6fe27ada93961b%3C/doaj%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-d136t-ed44498656cf61ff179ccc2011b85ea7029fa093ee7318a6645ca7bbffc2db1f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true