Effect of Essential Oils on the Oxyntopeptic Cells and Somatostatin and Ghrelin Immunoreactive Cells in the European Sea Bass ( Dicentrarchus labrax ) Gastric Mucosa

The current work was designed to assess the effect of feed supplemented with essential oils (EOs) on the histological features in sea bass's gastric mucosa. Fish were fed three diets: control diet (CTR), HERBAL MIX made with natural EOs (N-EOs), or HERBAL MIX made with artificial EOs obtained b...

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Bibliographic Details
Published in:Animals (Basel) 2021-11, Vol.11 (12), p.3401
Main Authors: Mazzoni, Maurizio, Lattanzio, Giulia, Bonaldo, Alessio, Tagliavia, Claudio, Parma, Luca, Busti, Serena, Gatta, Pier Paolo, Bernardi, Nadia, Clavenzani, Paolo
Format: Article
Language:English
Subjects:
Additives
Animal production
Animal welfare
Antibodies
Aquaculture
Automation
Bass
Diet
enteroendocrine cells
essential oil
Essential oils
Evaluation
Fatty acids
Feed additives
Food additives
Gastric mucosa
Gastrointestinal system
Gastrointestinal tract
Ghrelin
Histology
Hydrochloric acid
Immunomodulation
Mammals
Markers
Morphology
Na+/K+-exchanging ATPase
Na+K+-ATPase
Oils & fats
oxyntopeptic cells
Parietal cells
Peptides
Physiological responses
Sea bass
Somatostatin
Stomach
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Summary:The current work was designed to assess the effect of feed supplemented with essential oils (EOs) on the histological features in sea bass's gastric mucosa. Fish were fed three diets: control diet (CTR), HERBAL MIX made with natural EOs (N-EOs), or HERBAL MIX made with artificial EOs obtained by synthesis (S-EOs) during a 117-day feeding trial. Thereafter, the oxyntopeptic cells (OPs) and the ghrelin (GHR) and somatostatin (SOM) enteroendocrine cells (EECs) in the gastric mucosa were evaluated. The Na K -ATPase antibody was used to label OPs, while, for the EECs, anti-SOM and anti-GHR antibody were used. The highest density of OP immunoreactive (IR) area was in the CTR group (0.66 mm ± 0.1). The OP-IR area was reduced in the N-EO diet group (0.22 mm ± 1; CTR vs. N-EOs, < 0.005), while in the S-EO diet group (0.39 mm ± 1) a trend was observed. We observed an increase of the number of SOM-IR cells in the N-EO diet (15.6 ± 4.2) compared to that in the CTR (11.8 ± 3.7) (N-EOs vs. CTR; < 0.05), but not in the S-EOs diet. These observations will provide a basis to advance current knowledge on the anatomy and digestive physiology of this species in relation to pro-heath feeds.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11123401