Changes in Bull Semen Metabolome in Relation to Cryopreservation and Fertility

Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Fort...

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Bibliographic Details
Published in:Animals (Basel) 2020-06, Vol.10 (6), p.1065
Main Authors: Longobardi, Valentina, Kosior, Michal A, Pagano, Nunzia, Fatone, Gerardo, Staropoli, Alessia, Vassetti, Anastasia, Vinale, Francesco, Campanile, Giuseppe, Gasparrini, Bianca
Format: Article
Language:English
Subjects:
Acetyl-L-carnitine
Amino acids
Biomarkers
bull
Carnitine
Cattle
Chromatography
Cryopreservation
Deoxyribonucleic acid
DNA
Fertility
Fourier transforms
Glutamine
Glycerol
Glycine
Glycine betaine
In vitro fertilization
Infertility
LC–MS
Liquid chromatography
Lysophosphatidylcholine
Mass spectrometry
Mass spectroscopy
Metabolites
Morphology
Motility
Overexpression
Plasma
Reproductive technologies
Scientific imaging
Semen
Sperm
Spermatozoa
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Summary:Semen cryopreservation determines several sperm damages, including the loss of fertility-associated proteins. The purpose of the study was to compare the metabolite contents in bovine sperm and seminal plasma before and after cryopreservation, and between high- and low-fertility bulls in vitro. Forty-eight ejaculates, collected from eight bulls (six per bull), were analyzed by liquid chromatography-mass spectrometry. Cryopreservation resulted in an over-expression of lysophosphatidylcholine (0:0/18:2(9Z,12Z)) in seminal plasma. In addition, higher levels of glycine betaine and pyro-l-glutaminyl-l-glutamine were observed in cryopreserved compared to fresh spermatozoa. The fresh seminal plasma of high-fertility bulls showed an over-expression of l-acetylcarnitine, glycerol tripropanoate, 2,3-diacetoxypropyl stearate and glycerophosphocholine, and an under-expression of lysophosphatidylcholine and butyrylcarnitine, compared to low-fertility bulls. Higher levels of glycerophosphocholine and lysophosphatidylcholine (16:0/0:0) were recorded in fresh spermatozoa from high-fertility bulls. In high-fertility bulls, a greater content of glycerophosphocholine and lower levels of butyrylcarnitine, glycine betaine and l-carnitine were found in cryopreserved seminal plasma, and lower levels of glycine betaine were detected in cryopreserved spermatozoa. In conclusion, cryopreservation affects bovine semen metabolome at both plasmatic and cellular compartments, and metabolic profile differs between high- and low-fertility bulls.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani10061065