The Natural Compound Neobractatin Induces Cell Cycle Arrest by Regulating E2F1 and Gadd45α

The complexity and multi-target feature of natural compounds have made it difficult to elucidate their mechanism of action (MoA), which hindered the development of lead anticancer compounds to some extent. In this study, we applied RNA-Seq and GSEA transcriptome analysis to rapidly and efficiently e...

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Bibliographic Details
Published in:Frontiers in oncology 2019-07, Vol.9, p.654-654
Main Authors: Zheng, Zhaoqing, Wu, Man, Zhang, Juan, Fu, Wenwei, Xu, Naihan, Lao, Yuanzhi, Lin, Lan, Xu, Hongxi
Format: Article
Language:English
Subjects:
cell cycle
E2F1
GADD45α
GSEA
neobractatin
Oncology
RNA-Seq
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Summary:The complexity and multi-target feature of natural compounds have made it difficult to elucidate their mechanism of action (MoA), which hindered the development of lead anticancer compounds to some extent. In this study, we applied RNA-Seq and GSEA transcriptome analysis to rapidly and efficiently evaluate the anticancer mechanisms of neobractatin (NBT), a caged prenylxanthone isolated from the Chinese herb . We found that NBT exerted anti-proliferative effect on various cancer cells and caused both G1/S and G2/M arrest in synchronized cancer cells through its effects on the expression of E2F1 and GADD45α. The animal study further suggested that NBT could reduce tumor burden in HeLa xenograft model with no apparent toxicity. By demonstrating the biological effect of NBT, we provided evidences for further investigations of this novel natural compound with anticancer potential.
ISSN:2234-943X
2234-943X
DOI:10.3389/fonc.2019.00654