Direct and Indirect Antioxidant Effects of Selected Plant Phenolics in Cell-Based Assays

: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. : The cellular antioxidant assay (CAA) assay was employed for direct scaven...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2021-04, Vol.26 (9), p.2534
Main Authors: Treml, Jakub, Večeřová, Petra, Herczogová, Petra, Šmejkal, Karel
Format: Article
Language:English
Subjects:
Antioxidants
Assaying
CAA
Catalase
Cytotoxicity
Decomposition
Enzymes
Gene expression
glucose toxicity
Oxidative stress
Phenols
plant phenolics
Scavenging
superoxide dismutase
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Summary:: Oxidative stress is a key factor in the pathophysiology of many diseases. This study aimed to verify the antioxidant activity of selected plant phenolics in cell-based assays and determine their direct or indirect effects. : The cellular antioxidant assay (CAA) assay was employed for direct scavenging assays. In the indirect approach, the influence of each test substance on the gene and protein expression and activity of selected antioxidant enzymes was observed. One assay also dealt with activation of the Nrf2-ARE pathway. The overall effect of each compound was measured using a glucose oxidative stress protection assay. : Among the test compounds, acteoside showed the highest direct scavenging activity and no effect on the expression of antioxidant enzymes. It increased only the activity of catalase. Diplacone was less active in direct antioxidant assays but positively affected enzyme expression and catalase activity. Morusin showed no antioxidant activity in the CAA assay. Similarly, pomiferin had only mild antioxidant activity and proved rather cytotoxic. : Of the four selected phenolics, only acteoside and diplacone demonstrated antioxidant effects in cell-based assays.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26092534