Effects of metformin on human gingival fibroblasts: an in vitro study

To investigate the effects of metformin (MF) treatment on the matrix metalloproteinases (MMPs) and proinflammatory cytokines production from lipopolysaccharide (LPS) - stimulated human gingival fibroblasts (HGFs). HGFs were obtained from subcultures of biopsies from clinically healthy gingival tissu...

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Published in:BMC oral health 2023-05, Vol.23 (1), p.292-292, Article 292
Main Authors: Alshibani, Nouf, AlKattan, Reem, Allam, Eman, Alshehri, Fahad A, Shalabi, Manal Matouq, Almuhanna, Nada, Almarshad, Houriah, Aljamili, Anfal
Format: Article
Language:English
Subjects:
Analysis
Antidiabetics
Bacteria
Biopsy
Care and treatment
Cells
Cells, Cultured
Cytokines
Cytotoxicity
Diabetes
Diagnosis
Disease
Dosage and administration
Enzymes
Fibroblasts
Fibroblasts - metabolism
Gelatinase A
Gelatinase B
Gingiva
Gingiva - metabolism
Glucose
Gum disease
Health care
Human gingival fibroblasts
Humans
Immune response
Inflammation
Interleukin 8
Interleukin-8 - metabolism
Interstitial collagenase
Lipopolysaccharides
Lipopolysaccharides - metabolism
Lipopolysaccharides - pharmacology
Matrix metalloproteinase
Matrix Metalloproteinase 1 - analysis
Matrix Metalloproteinase 1 - metabolism
Matrix Metalloproteinase 1 - pharmacology
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 8
Matrix metalloproteinases
Metformin
Metformin - metabolism
Metformin - pharmacology
Microbiota
Neutrophil collagenase
Pathogenesis
Periodontal disease
Periodontal diseases
Porphyromonas gingivalis
Statistical analysis
Citations: Items that this one cites
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Summary:To investigate the effects of metformin (MF) treatment on the matrix metalloproteinases (MMPs) and proinflammatory cytokines production from lipopolysaccharide (LPS) - stimulated human gingival fibroblasts (HGFs). HGFs were obtained from subcultures of biopsies from clinically healthy gingival tissues of patients undergoing oral surgeries. Cell cytotoxicity assay was used to determine the effect of different concentrations of MF on viability of HGFs. HGFs were then incubated and treated with different concentrations of MF and Porphyromonas gingivais (Pg) LPS. MMP-1, MMP-2, MMP-8, MMP-9, IL-1β, and IL-8 expression analysis was performed using xMAP technology (Luminex 200, Luminex, Austin, TX, USA). Student's t-test for a single sample was used to compare the mean values of the study groups with the control value. A p-value of
ISSN:1472-6831
1472-6831
DOI:10.1186/s12903-023-02978-0