Legume-nodulating rhizobia are widespread in soils and plants across the island of O'ahu, Hawai'i

• Published in: PloS one 2023-09, Vol.18 (9), p.e0291250
• Main Authors: Abe, Jonathan N. A, Dhungana, Ishwora, Nguyen, Nhu H
• Format: Article
• Language: English
• Subjects: Beans; Legumes; Management; Microbiota (Symbiotic organisms); Mimosaceae; Organic farming; Production management; Properties; Soils
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0291250

Legumes and their interaction with rhizobia represent one of the most well-characterized symbioses that are widespread across both natural and agricultural environments. However, larger distribution patterns and host associations on isolated Pacific islands with many native and introduced hosts have not been well-documented. Here, we used molecular and culturing techniques to characterize rhizobia from soils and 24 native and introduced legume species on the island of O'ahu, Hawai'i. We chose two of these isolates to inoculate an endemic legume tree, Erythina sandwicensis to measure nodulation potentials and host benefits. We found that all rhizobia genera can be found in the soil, where only Cupriavidus was found at all sites, although at lower abundance relative to other more common genera such as Rhizobium (and close relatives), Bradyzhizobium, and Devosia. Bradyrhizobium was the most common nodulator of legumes, where the strain Bradyrhizobium sp. strain JA1 is a generalist capable of forming nodules on nine different host species, including two native species. In greenhouse nursery inoculations, the two different Bradyrhizobium strains successfully nodulate the endemic E. sandwicensis; both strains equally and significantly increased seedling biomass in nursery inoculations. Overall, this work provides a molecular-based framework in which to study potential native and introduced rhizobia on one of the most isolated archipelagos on the planet.