Bioleaching of Chalcopyrite by a New Strain Leptospirillum ferrodiazotrophum Ksh-L Isolated from a Dump-Bioleaching System of Kashen Copper-Molybdenum Mine

A new strain of Leptospirillum sp. Ksh-L was isolated from a dump-bioleaching system of the Kashen copper-molybdenum mine (South Caucasus). Ksh-L is an obligate chemolithoautotroph, capable of oxidizing ferrous iron (Fe2+). Cells are Gram-negative and vibrio- or spirillum-shaped of a 0.5–3 µm size....

Full description

Saved in:
Bibliographic Details
Published in:Minerals (Basel) 2024-01, Vol.14 (1), p.26
Main Authors: Khachatryan, Anna, Vardanyan, Narine, Willscher, Sabine, Sevoyan, Garegin, Zhang, Ruiyong, Vardanyan, Arevik
Format: Article
Language:English
Subjects:
Bacteria
Bacterial leaching
Biofilms
bioleaching of sulfide minerals
Cell culture
Cell division
Chalcopyrite
characterization
Copper
Cytochrome
Cytoplasm
Gene sequencing
Genes
Genomes
Genomics
Iron
isolation
Leaching
Leptospirillum ferrodiazotrophum
Metabolism
Metals
Microorganisms
Minerals
Molybdenum
Morphology
Nitrogen
Nucleotide sequence
Oxidation
phylogenetic analysis
Phylogenetics
Phylogeny
rRNA 16S
Signal transduction
Zinc
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new strain of Leptospirillum sp. Ksh-L was isolated from a dump-bioleaching system of the Kashen copper-molybdenum mine (South Caucasus). Ksh-L is an obligate chemolithoautotroph, capable of oxidizing ferrous iron (Fe2+). Cells are Gram-negative and vibrio- or spirillum-shaped of a 0.5–3 µm size. The optimal conditions for the growth are 35 °C and pH 1.6–1.8. Cu2+ and Zn2+ have different effects on the oxidizing ability of the Leptospirillum sp. Ksh-L culture depending on the phase of growth and concentration of Fe2+. Under the conditions of gradually increasing the concentration of copper in the medium, during 4–5 successive subculturing experiments, it was possible to obtain an adapted culture of Leptospirillum sp. Ksh-L, capable of growing in the medium in the presence of up to 400 mM Cu2+. A bioleaching experiment indicates that Ksh-L can efficiently oxidize chalcopyrite. However, the bioleaching of copper from chalcopyrite by Leptospirillum ferrodiazotropum Ksh-L increased about 1.8 times in association with At. thiooxidans ATCC 19377. Phylogenetic analysis based on 16S rRNA gene sequences (GenBank ID ON226845) shows that strain Ksh-L forms a single cluster into Group III. The strain possesses 99.59%, 99.52%, and 96.60% sequence similarity with the strains YTW-96-06, YTW-66-06, and Leptospirillum ferrodiazotrophum 5C in Group III, respectively.
ISSN:2075-163X
2075-163X
DOI:10.3390/min14010026