Extraction and Analysis of Methadone in Exhaled Breath Condensate Using a Validated LC-UV Method

A combined microextraction and separation method is presented for the determination of methadone in exhaled breath condensate (EBC) which is a promising non-invasive biological component for monitoring drug concentrations. In this work, dispersive liquid-liquid microextraction (DLLME) and ultrasonic...

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Bibliographic Details
Published in:Journal of pharmacy & pharmaceutical sciences 2015-01, Vol.18 (2), p.207-219
Main Authors: Khoubnasabjafari, Maryam, Ansarin, Khalil, Jouyban-Gharamaleki, Vahid, Panahi-Azar, Vahid, Shayanfar, Ali, Mohammadzadeh, Laya, Jouyban, Abolghasem
Format: Article
Language:English
Subjects:
Adult
Breath Tests - methods
Chromatography, Liquid
Female
Healthy Volunteers
Humans
Liquid Phase Microextraction
Male
Methadone - analysis
Methadone - isolation & purification
Middle Aged
Molecular Structure
Ultrasonics
Ultraviolet Rays
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Summary:A combined microextraction and separation method is presented for the determination of methadone in exhaled breath condensate (EBC) which is a promising non-invasive biological component for monitoring drug concentrations. In this work, dispersive liquid-liquid microextraction (DLLME) and ultrasonic liquid-liquid microextraction (ULLME) procedure coupled with a validated liquid chromatography method were used for analysis of methadone in EBC collected using an in-house cold trap setup. The method has been validated according to the FDA guidelines using EBC-spiked samples and tested on a number of EBC samples collected from patients. The best DLLME conditions involved the use of a disperser solvent of methanol (1 mL), extraction solvent of chloroform (200 mL), EBC sample pH of 10.0 and centrifugation at 6000 rpm for 5 minutes. The conditions for ULLME were 150 mL of chloroform and the samples were sonicated for 4 minutes. The method was validated over the concentration range of 0.5-10 mg/L-1 in EBC. Inter- and intra-day precision and accuracy were less than 5 % where the acceptable levels are less than 20%. Furthermore, the validated method was successfully applied for the determination of methadone in patients' EBC samples. The outcomes indicate that the developed LC-UV combined with DLLME and/or ULLME extraction methods can be employed for the extraction and separation of methadone in EBC samples.
ISSN:1482-1826
1482-1826
DOI:10.18433/J3WK65