Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens

Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine, and the inactivated vaccine is used to prevent and control SVA infection. To develop a new chromatography strategy for the purification and concentration of SVA vaccine antigens, we inserted a 6×His-tag at the VP1 C-termin...

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Bibliographic Details
Published in:Vaccines (Basel) 2022-01, Vol.10 (2), p.170
Main Authors: Fan, Junhao, Xiao, Peiyu, Kong, Dongni, Liu, Xinran, Meng, Liang, An, Tongqing, Cai, Xuehui, Wang, Haiwei, Yu, Li
Format: Article
Language:English
Subjects:
Affinity
Antibodies
antigen purification
Antigenicity
Antigens
Deactivation
Foot & mouth disease
Growth kinetics
His-tagged virus
Hogs
Immunogenicity
Insertion
Neutralizing
Product development
Purification
Rabbits
Senecavirus A
Surface stability
Vaccines
Viruses
VP1 protein
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Summary:Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine, and the inactivated vaccine is used to prevent and control SVA infection. To develop a new chromatography strategy for the purification and concentration of SVA vaccine antigens, we inserted a 6×His-tag at the VP1 C-terminal of the SVA/HLJ/CHA/2016 in an infectious clone to rescue a His-tagged SVA. The constructed and rescued recombinant virus, named as rSVA-His, exhibited similar growth kinetics to that of its parental virus. In addition, the expression of a 6×His-tag on the surface of SVA showed genetic stability in cell passages in vitro, which allowed one-step purification of SVA antigens by Ni affinity columns. Furthermore, the immunogenicity of the inactivated rSVA-His was evaluated by inoculating rabbits and detecting neutralizing antibodies. The animals receiving two doses of the inactivated rSVA-His emulsified with oil adjuvant developed a high titer of neutralizing antibodies, indicating that SVA VP1 is tolerant to His-tag insertion without detriment to its antigenicity. In summary, the constructed 6×His-tagged SVA may offer a feasible approach to the affinity purification and concentration of antigens in the process of SVA inactivated vaccine production.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines10020170