Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants

Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. LHCG...

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Bibliographic Details
Published in:Reproductive biology and endocrinology 2011-05, Vol.9 (1), p.64-64, Article 64
Main Authors: Chambers, Anne E, Stanley, Paul F, Randeva, Harpal, Banerjee, Subhasis
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
CHO Cells
Cricetinae
Cricetulus
Female
Gonadotropin releasing hormone
Humans
Organ Culture Techniques
Physiological aspects
Placenta
Placenta - metabolism
Placenta - secretion
Pregnancy
Receptors, LH - genetics
Receptors, LH - metabolism
Recombinant proteins
Secretory Vesicles - metabolism
Solubility
Transfection
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Summary:Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. Secretion was minimal up to 72h but by 96h 24-37% of the LHCGR had been released into the culture medium. The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. Secretion was independent of recombinant size and was mediated via structurally defined membrane vesicles (50-150nm). Similarly cultured human early pregnancy placental explants also released LHCGR via microvesicles. These studies provide the first experimental evidence of the possible mechanistic basis of the secretion of LHCGR.
ISSN:1477-7827
1477-7827
DOI:10.1186/1477-7827-9-64