Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus

Biofilm formation of Vibrio vulnificus is initiated by adherence of flagellated cells to surfaces, and then flagellum-driven motility is not necessary during biofilm maturation. Once matured biofilms are constructed, cells become flagellated and swim to disperse from biofilms. As a consequence, time...

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Bibliographic Details
Published in:NPJ biofilms and microbiomes 2021-04, Vol.7 (1), p.32-32, Article 32
Main Authors: Jung, You-Chul, Lee, Mi-Ae, Kim, Han-Shin, Lee, Kyu-Ho
Format: Article
Language:English
Subjects:
631/326/1320
631/326/46
Biofilms
Biomedical and Life Sciences
Developmental stages
Flagella
Flagellin
Life Sciences
Maturation
Medical Microbiology
Methionine
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Motility
Vibrio vulnificus
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Summary:Biofilm formation of Vibrio vulnificus is initiated by adherence of flagellated cells to surfaces, and then flagellum-driven motility is not necessary during biofilm maturation. Once matured biofilms are constructed, cells become flagellated and swim to disperse from biofilms. As a consequence, timely regulations of the flagellar components’ expression are crucial to complete a biofilm life-cycle. In this study, we demonstrated that flagellins’ production is regulated in a biofilm stage-specific manner, via activities of a protease DegQ and a chaperone FlaJ. Among four flagellin subunits for V. vulnificus filament, FlaC had the highest affinities to hook-associated proteins, and is critical for maturating flagellum, showed the least susceptibility to DegQ due to the presence of methionine residues in its DegQ-sensitive domains, ND1 and CD0. Therefore, differential regulation by DegQ and FlaJ controls the cytoplasmic stability of flagellins, which further determines the motility-dependent, stage-specific development of biofilms.
ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-021-00206-7