Variability in Susceptibility to Type I Interferon Response and Subgenomic RNA Accumulation Between Clinical Isolates of Dengue and Zika Virus From Oaxaca Mexico Correlate With Replication Efficiency in Human Cells and Disease Severity

Dengue and Zika viruses cocirculate annually in endemic areas of Mexico, causing outbreaks of different magnitude and severity every year, suggesting a continuous selection of Flavivirus variants with variable phenotypes of transmissibility and virulence. To evaluate if Flavivirus variants with diff...

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Published in:Frontiers in cellular and infection microbiology 2022-06, Vol.12, p.890750-890750
Main Authors: Castro-Jiménez, Tannya Karen, Gómez-Legorreta, Laura Cristina, López-Campa, Laura Alejandra, Martínez-Torres, Valeria, Alvarado-Silva, Marcos, Posadas-Mondragón, Araceli, Díaz-Lima, Nallely, Angulo-Mendez, Hilda Arcelia, Mejía-Domínguez, Nancy R., Vaca-Paniagua, Felipe, Ávila-Moreno, Federico, García-Cordero, Julio, Cedillo-Barrón, Leticia, Aguilar-Ruíz, Sergio Roberto, Bustos-Arriaga, José
Format: Article
Language:English
Subjects:
Cellular and Infection Microbiology
dengue
interferons
isolates
sfRNA
Zika
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Summary:Dengue and Zika viruses cocirculate annually in endemic areas of Mexico, causing outbreaks of different magnitude and severity every year, suggesting a continuous selection of Flavivirus variants with variable phenotypes of transmissibility and virulence. To evaluate if Flavivirus variants with different phenotypes cocirculate during outbreaks, we isolated dengue and Zika viruses from blood samples of febrile patients from Oaxaca City during the 2016 and 2019 epidemic years. We compared their replication kinetics in human cells, susceptibility to type I interferon antiviral response, and the accumulation of subgenomic RNA on infected cells. We observed correlations between type I interferon susceptibility and subgenomic RNA accumulation, with high hematocrit percentage and thrombocytopenia. Our results suggest that Flaviviruses that cocirculate in Oaxaca, Mexico, have variable sensitivity to the antiviral activity of type I interferons, and this phenotypic trait correlates with the severity of the disease.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2022.890750