In planta proximity dependent biotin identification (BioID)

Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate...

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Bibliographic Details
Published in:Scientific reports 2018-06, Vol.8 (1), p.9212-8, Article 9212
Main Authors: Khan, Madiha, Youn, Ji-Young, Gingras, Anne-Claude, Subramaniam, Rajagopal, Desveaux, Darrell
Format: Article
Language:English
Subjects:
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Biotin
Datasets
Humanities and Social Sciences
Localization
Mammalian cells
Mass spectrometry
multidisciplinary
Plant tissues
Protein expression
Proteins
Proteomics
R&D
Research & development
Science
Science (multidisciplinary)
Scientific imaging
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Summary:Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana , thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-27500-3