Clam Genome Sequence Clarifies the Molecular Basis of Its Benthic Adaptation and Extraordinary Shell Color Diversity

• Published in: iScience 2019-09, Vol.19, p.1225-1237
• Main Authors: Yan, Xiwu, Nie, Hongtao, Huo, Zhongming, Ding, Jianfeng, Li, Zhenzhen, Yan, Lulu, Jiang, Liwen, Mu, Zhengqiang, Wang, Huamin, Meng, Xiangyu, Chen, Peng, Zhou, Mengyan, Rbbani, Md. Golam, Liu, Guangjian, Li, Dongdong
• Format: Article
• Language: English
• Subjects: Biological Sciences; Genetics; Genomics
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2019.08.049

Ruditapes philippinarum is an economically important bivalve with remarkable diversity in its shell coloration patterns. In this study, we sequenced the whole genome of the Manila clam and investigated the molecular basis of its adaptation to hypoxia, acidification, and parasite stress with transcriptome sequencing and an RNA sequence analysis of different tissues and developmental stages to clarify these major issues. A number of immune-related gene families are expanded in the R. philippinarum genome, such as TEP, C3, C1qDC, Hsp70, SABL, and lysozyme, which are potentially important for its stress resistance and adaptation to a coastal benthic life. The transcriptome analyses demonstrated the dynamic and orchestrated specific expression of numerous innate immune-related genes in response to experimental challenge with pathogens. These findings suggest that the expansion of immune- and stress-related genes may play vital roles in resistance to adverse environments and has a profound effect on the clam's adaptation to benthic life. [Display omitted] •We present a new genome assembly of the Manila clam Ruditapes philippinarum•Analysis of gene family expansions and transcriptome characterization were conducted•Tyr and mitf genes were potentially involved in shell color patterns of Manila clam•Expansion of GPCRs and immune-related genes were found in R. philippinarum Biological Sciences; Genetics; Genomics