Effect and Mechanism of Theaflavins on Fluoride Transport and Absorption in Caco-2 Cells

This paper investigated the effect and mechanism of theaflavins (TFs) on fluoride (F ) uptake and transport in the Caco-2 cell model through structural chemistry and transcriptome analysis. The results showed that the four major TFs (TF, TF3G, TF3'G and TFDG) at a 150 μg/mL concentration could...

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Bibliographic Details
Published in:Foods 2023-04, Vol.12 (7), p.1487
Main Authors: Fan, Yueqin, Lei, Zhendong, Huang, Jiasheng, Su, Dan, Ni, Dejiang, Chen, Yuqiong
Format: Article
Language:English
Subjects:
Absorption
Adenocarcinoma
Antioxidants
Benzene
Bioavailability
caco-2 cells
Calorimetry
Cancer cells
Cell culture
Cell permeability
Chemical properties
Covalent bonds
Efflux
Experiments
fluoride
Fluorides
Food science
Gene expression
Genes
Genetic aspects
Mechanical properties
mechanism
Membrane permeability
Metabolism
Morphology
Permeability
Pharmaceutical research
Phosphatase
Polyphenols
Raman spectroscopy
Tea
Theaflavins
Titration
Titration calorimetry
Transcriptomes
transport and absorption
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Summary:This paper investigated the effect and mechanism of theaflavins (TFs) on fluoride (F ) uptake and transport in the Caco-2 cell model through structural chemistry and transcriptome analysis. The results showed that the four major TFs (TF, TF3G, TF3'G and TFDG) at a 150 μg/mL concentration could all significantly decrease F transport in Caco-2 cells after 2 h of treatment and, at 2 μg/mL F concentration, the F transport was more inclined to efflux. During transport, the F retention in Caco-2 cells was significantly increased by TF3G while it was clearly decreased by TF. The interaction between TFs and F was analyzed by Raman spectroscopy and isothermal titration calorimetry, and F was shown to affect the π bond vibration on the benzene ring of TFs, thus influencing their stability. Additionally, F showed weak binding to TF3G, TF3'G and TFDG, which may inhibit F transport and absorption in the Caco-2 cell line. Transcriptome and RT-PCR analysis identified three key differentially expressed genes related to cell permeability, and TFs can be assumed to mediate F transport by regulating the expression of permeability-related genes to change cell monolayer permeability and enhance cell barrier function; however, this needs to be further elucidated in future studies.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods12071487