Effects of Lectins from Alpinia purpurata Inflorescence (ApuL) and Schinus terebinthifolia Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells

Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from Alpinia purpurata inflorescence (ApuL) and Schinus terebinthifolia leaf (SteLL) on the viability of human leukemia cells (K562, ch...

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Published in:Macromol 2023-06, Vol.3 (2), p.290-302
Main Authors: de Santana Brito, Jéssica, de Oliveira Marinho, Amanda, de Siqueira Patriota, Leydianne Leite, Gaião, Wyndly Daniel Cardoso, Torres, Diego José Lira, Paiva, Patrícia Maria Guedes, de Lorena, Virgínia Maria Barros, Rodrigues, Cláudio Gabriel, da Silva, Márcia Bezerra, Napoleão, Thiago Henrique
Format: Article
Language:English
Subjects:
anticancer
Bone marrow
Brazilian pepper tree
Cancer therapies
carbohydrate-binding proteins
Carbohydrates
Cytotoxicity
Glycoproteins
Lectins
Leukemia
Proteins
Quantum dots
red ginger
Stem cells
Tumor necrosis factor-TNF
Umbilical cord
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Summary:Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from Alpinia purpurata inflorescence (ApuL) and Schinus terebinthifolia leaf (SteLL) on the viability of human leukemia cells (K562, chronic myeloid leukemia; JURKAT, acute lymphoblastic leukemia) and mesenchymal stem cells (MSCs) from human umbilical cords. In addition, possible immunomodulatory effects of ApuL and SteLL on MSCs were assessed by determining cytokine levels in cultures. ApuL reduced the viability of JURKAT cells (IC50: 12.5 μg/mL), inducing both apoptosis and necrosis. For K562 cells, ApuL at 50 µg/mL caused a decrease in viability, but of only 8.8%. Conversely, SteLL exerted a cytotoxic effect on K562 (IC50: 6.0 μg/mL), inducing apoptosis, while it was not cytotoxic to JURKAT. ApuL and SteLL (0.19–100 μg/mL) did not decrease MSCs viability. Treatment with ApuL strongly suppressed (99.5% reduction) the release of IL-6 by MSCs. SteLL also reduced the levels of this cytokine in culture supernatant. In conclusion, ApuL and SteLL showed potential to reduce the viability of leukemia cells, as well as immunomodulatory effect on MSCs without being toxic to them. These biological properties can be explored biomedically and biotechnologically in the future.
ISSN:2673-6209
2673-6209
DOI:10.3390/macromol3020018