A spontaneously immortalized muscle stem cell line (EfMS) from brown-marbled grouper for cell-cultured fish meat production

Lacking of suitable fish muscle stem cell line has greatly hindered the fabrication of cell-cultured fish meat. Here, we established and characterized a spontaneously immortalized marine fish muscle stem cell line (EfMS) from brown-marbled grouper ( Epinephelus fuscoguttatus ), which could actively...

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Bibliographic Details
Published in:Communications biology 2024-12, Vol.7 (1), p.1697-15, Article 1697
Main Authors: Xue, Ting, Zheng, Hongwei, Zhao, Yaqi, Zhao, Zhenxin, Wang, Jinwu, Zhang, Yue, Li, Yaru, Wang, Song, Liu, Yongliang, Xue, Changhu, Guo, Huarong
Format: Article
Language:English
Subjects:
13
13/100
631/532/2439
631/61/2320
706/1143
Animals
Bass
Biomedical and Life Sciences
Cell culture
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Epinephelus fuscoguttatus
Life Sciences
Meat
Meat production
Muscle Development
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Myogenesis
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Taurine
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Summary:Lacking of suitable fish muscle stem cell line has greatly hindered the fabrication of cell-cultured fish meat. Here, we established and characterized a spontaneously immortalized marine fish muscle stem cell line (EfMS) from brown-marbled grouper ( Epinephelus fuscoguttatus ), which could actively proliferate with good genetic stability and well maintain the stemness of myogenesis potential for over 50 passages. Taurine was found to be able to serve as a substitute of fish muscle extract in maintaining stemness. The EfMS cells could be efficiently induced to myogenic differentiation or adipogenic trans-differentiation in both 2D and 3D culture systems. Using edible 3D microcarriers, we produced 0.65 g fat-free and 1.47 g fat-containing cell-cultured fish meat in 8 days. The scaffold-free cell-cultured fish meat exhibited a much higher content of flavory amino acids than natural fish. Together, EfMS cell line can serve as an ideal seed cell line for the production of cell-cultured fish meat. Establishment and characterization of a spontaneously immortalized fish muscle stem cell line (EfMS) from brown-marbled grouper with undiminished stemness and differentiation potential for the production of cell-cultured fish meat.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-024-07400-1