The Dynamics of Synthesis and Localization of Jumbo Phage RNA Polymerases inside Infected Cells

A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) by two RNAPs encoded by the phage. The virio...

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Bibliographic Details
Published in:Viruses 2023-10, Vol.15 (10), p.2096
Main Authors: Antonova, Daria, Belousova, Viktoriia V, Zhivkoplias, Erik, Sobinina, Mariia, Artamonova, Tatyana, Vishnyakov, Innokentii E, Kurdyumova, Inna, Arseniev, Anatoly, Morozova, Natalia, Severinov, Konstantin, Khodorkovskii, Mikhail, Yakunina, Maria V
Format: Article
Language:English
Subjects:
Antibodies
Bacteriophages
Cloning
DNA-directed RNA polymerase
Enzymes
Genes
Genetic aspects
Genetic transcription
Genomes
giant phages
Glycerol
Host-virus relationships
Infections
jumbo phages
Life sciences
Localization
Microscopy
phage nucleus
Phages
phiKZ
Physiological aspects
Proteins
RNA phages
RNA polymerases
Structure
Synthesis
transcription regulation
virion RNA-polymerase
Virions
Virus research
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Summary:A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) by two RNAPs encoded by the phage. The virion RNAP (vRNAP) transcribes early viral genes and must be injected into the cell with phage DNA. The non-virion RNAP (nvRNAP) is composed of early gene products and transcribes late viral genes. In this work, the dynamics of phage RNAPs localization during phage phiKZ infection were studied. We provide direct evidence of PhiKZ vRNAP injection in infected cells and show that it is excluded from the phage nucleus. The nvRNAP is synthesized shortly after the onset of infection and localizes in the nucleus. We propose that spatial separation of two phage RNAPs allows coordinated expression of phage genes belonging to different temporal classes.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15102096