Aloe-Emodin Overcomes Anti-Cancer Drug Resistance to Temozolomide and Prevents Colony Formation and Migration in Primary Human Glioblastoma Cell Lines NULU and ZAR

Glioblastoma, the most dangerous and aggressive type of CNS tumor, appears resistant to many chemotherapy drugs. In the patient-derived glioma cell lines NULU and ZAR, which exhibit drug-resistant phenotypes, we investigated the effect of combined AE (Aloe-emodin) and TMZ (temozolomide) and found a...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2023-08, Vol.28 (16), p.6024
Main Authors: Staffieri, Sabrina, Russo, Veronica, Oliva, Maria Antonietta, Alborghetti, Marika, Russo, Miriam, Arcella, Antonietta
Format: Article
Language:English
Subjects:
aloe-emodin (AE)
Alzheimer's disease
Apoptosis
autophagy
Brain cancer
Brain research
Cancer
Cancer therapies
Cell growth
Chemotherapy
Comparative analysis
Computer software industry
Cytotoxicity
DNA methylation
DNA repair
Drug resistance
Drug therapy
Enzymes
Genotype & phenotype
glioblastoma (GBM)
Glioblastoma multiforme
Glioma
Medical prognosis
MGMT
Patients
Phytochemicals
Prevention
Protein expression
Proteins
Radiation therapy
Stem cells
Temozolomide
temozolomide (TMZ)
Toxicity
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Summary:Glioblastoma, the most dangerous and aggressive type of CNS tumor, appears resistant to many chemotherapy drugs. In the patient-derived glioma cell lines NULU and ZAR, which exhibit drug-resistant phenotypes, we investigated the effect of combined AE (Aloe-emodin) and TMZ (temozolomide) and found a significant additive inhibitory effect on cell growth and a promising cytotoxic effect on both cell lines compared to treatment with single agents. We also examined the effect of combined AE and TMZ treatment on the drug-resistance protein MGMT. The results suggest that using AE combined with traditional drugs restores drug resistance in both primary resistant cell lines (NULU and ZAR). Furthermore, migration assays and scratch tests showed that the combined use of AE and TMZ can slow down the colony formation and migration of glioblastoma cells. These convincing results suggest that AE could be a natural adjuvant agent to potentiate the effects of traditional drugs (TMZ) and overcome drug resistance in glioblastoma cells.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28166024