A re-annotation of the Anopheles darlingi mobilome

The mobilome, portion of the genome composed of transposable elements (TEs), of Anopheles darlingi was described together with the genome of this species. Here, this mobilome was revised using similarity and de novo search approaches. A total of 5.6% of the A. darlingi genome is derived of TEs. Clas...

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Bibliographic Details
Published in:Genetics and molecular biology 2019-01, Vol.42 (1), p.125-131
Main Authors: Diesel, Jose Francisco, Ortiz, Mauro F, Marinotti, Osvaldo, Vasconcelos, Ana Tereza R, Loreto, Elgion L S
Format: Article
Language:English
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Anopheles darlingi
BIOCHEMISTRY & MOLECULAR BIOLOGY
evolvability
Genetic transformation
GENETICS & HEREDITY
Genomes
Genomics and Bioinformatics
mosquitoes
Transposable elements
Transposons
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Summary:The mobilome, portion of the genome composed of transposable elements (TEs), of Anopheles darlingi was described together with the genome of this species. Here, this mobilome was revised using similarity and de novo search approaches. A total of 5.6% of the A. darlingi genome is derived of TEs. Class I gypsy and copia were the most abundant superfamilies, corresponding to 22.36% of the mobilome. Non-LTR elements of the R1 and Jockey superfamilies account for 11% of the TEs. Among Class II TEs, the mariner superfamily is the most abundant (16.01%). Approximately 87% of the A. darlingi mobilome consist of short, truncated and/or degenerated copies of TEs. Only three retrotransposons, two belonging to gypsy and one to copia superfamilies, are putatively active elements. Only one Class II element, belonging to the mariner superfamily, is putatively active, having 12 copies in the genome. The TE landscape of A. darlingi is formed primarily by degenerated elements and, therefore, somewhat stable. Future applications of TE-based vectors for genetic transformation of A. darlingi should take into consideration mariner and piggyBac transposons, because full length and putatively active copies of these elements are present in its genome.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-gmb-2017-0300