Red-Shifted Environmental Fluorophores and Their Use for the Detection of Gram-Negative Bacteria

Two novel, water-soluble, merocyanine fluorophores were readily prepared by microwave-assisted synthesis. Full optical characterization was performed in a series of protic and aprotic solvents, and the dyes displayed fluorescence in the red region with up to a 20-fold decrease in brightness in water...

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Bibliographic Details
Published in:Chemosensors 2021, Vol.9 (6), p.117
Main Authors: Megia-Fernandez, Alicia, Klausen, Maxime, Mills, Bethany, Brown, Gillian E., McEwan, Heather, Finlayson, Neil, Dhaliwal, Kevin, Bradley, Mark
Format: Article
Language:English
Subjects:
Acids
Antibiotics
Antimicrobial agents
Bacteria
bacterial detection
Bacterial infections
Chemical compounds
Chromatography
Drug resistance
Dyes
environmental fluorophores
Fluorescence
fluorescent labelling
Fluorophores
Gram-negative bacteria
Gram-positive bacteria
Infections
Mass spectrometry
merocyanines
optical imaging
Optical properties
Pathogens
Scientific imaging
solvato-fluorogenicity
Solvents
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Summary:Two novel, water-soluble, merocyanine fluorophores were readily prepared by microwave-assisted synthesis. Full optical characterization was performed in a series of protic and aprotic solvents, and the dyes displayed fluorescence in the red region with up to a 20-fold decrease in brightness in water, demonstrating a strong environmental sensitivity hereby termed as solvato-fluorogenicity (to distinguish from solvatochromism). Shorter fluorescent lifetimes were also measured in water, which confirmed this character. These dyes were conjugated to a modified polymyxin scaffold that allowed fluorescence “switch-on” upon binding to Gram-negative bacterial membranes, and selective fluorescence detection of bacteria in a wash-free protocol.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors9060117