Direct Comparison of the Lowest Effect Concentrations of Mutagenic Reference Substances in Two Ames Test Formats

The Ames assay is the standard assay for identifying DNA-reactive genotoxic substances. Multiple formats are available and the correct choice of an assay protocol is essential for achieving optimal performance, including fit for purpose detection limits and required screening capacity. In the presen...

Full description

Saved in:
Bibliographic Details
Published in:Toxics (Basel) 2021-06, Vol.9 (7), p.152
Main Authors: Rainer, Bernhard, Pinter, Elisabeth, Prielinger, Lukas, Coppola, Chiara, Marin-Kuan, Maricel, Schilter, Benoit, Apprich, Silvia, Tacker, Manfred
Format: Article
Language:English
Subjects:
Ames assay
Ames test
Aroclor 1254
Assaying
bacterial reverse mutation
Chemicals
complex mixtures
Detection limits
food contact materials
Format
Genotoxic chemicals
Genotoxicity
Laboratories
Low concentrations
Metabolism
mutagenicity
Naphthoflavone
PCB
Phenobarbital
Polychlorinated biphenyls
Screening
Solvents
Suppliers
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Ames assay is the standard assay for identifying DNA-reactive genotoxic substances. Multiple formats are available and the correct choice of an assay protocol is essential for achieving optimal performance, including fit for purpose detection limits and required screening capacity. In the present study, a comparison of those parameters between two commonly used formats, the standard pre-incubation Ames test and the liquid-based Ames MPF™, was performed. For that purpose, twenty-one substances with various modes of action were chosen and tested for their lowest effect concentrations (LEC) with both tests. In addition, two sources of rat liver homogenate S9 fraction, Aroclor 1254-induced and phenobarbital/β-naphthoflavone induced, were compared in the Ames MPF™. Overall, the standard pre-incubation Ames and the Ames MPF™ assay showed high concordance (>90%) for mutagenic vs. non-mutagenic compound classification. The LEC values of the Ames MPF™ format were lower for 17 of the 21 of the selected test substances. The S9 source had no impact on the test results. This leads to the conclusion that the liquid-based Ames MPF™ assay format provides screening advantages when low concentrations are relevant, such as in the testing of complex mixtures.
ISSN:2305-6304
2305-6304
DOI:10.3390/toxics9070152