Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

• Published in: International journal of molecular sciences 2024-05, Vol.25 (9), p.4625
• Main Authors: Miljuš, Goran, Penezić, Ana, Pažitná, Lucia, Gligorijević, Nikola, Baralić, Marko, Vilotić, Aleksandra, Šunderić, Miloš, Robajac, Dragana, Dobrijević, Zorana, Katrlík, Jaroslav, Nedić, Olgica
• Format: Article
• Language: English
• Subjects: Adult; Aged; Anemia; Chronic kidney failure; Cytochemistry; end-stage kidney disease; Female; Glycosylation; Health aspects; Hemodialysis; Humans; Iron; Iron - metabolism; Kidney diseases; Kidney Failure, Chronic - metabolism; Kidney Failure, Chronic - therapy; lectin microarray; Lectins; Male; Medical research; Medicine, Experimental; Metabolism; Middle Aged; Peritoneal Dialysis; Physiological aspects; Proteins; Receptors, Transferrin - metabolism; structure; Transferrin; Transferrin - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25094625

Chronic kidney disease (CKD) is a global health concern affecting approximately one billion individuals worldwide. End-stage kidney disease (ESKD), the most severe form of CKD, is often accompanied by anemia. Peritoneal dialysis (PD), a common treatment for ESKD, utilizes the peritoneum for solute transfer but is associated with complications including protein loss, including transferrin (Tf) a key protein involved in iron transport. This study investigated Tf characteristics in ESKD patients compared to healthy individuals using lectin microarray, spectroscopic techniques and immunocytochemical analysis to assess Tf interaction with transferrin receptors (TfRs). ESKD patients exhibited altered Tf glycosylation patterns, evidenced by significant changes in lectin reactivity compared to healthy controls. However, structural analyses revealed no significant differences in the Tf secondary or tertiary structures between the two groups. A functional analysis demonstrated comparable Tf-TfR interaction in both PD and healthy samples. Despite significant alterations in Tf glycosylation, structural integrity and Tf-TfR interaction remained preserved in PD patients. These findings suggest that while glycosylation changes may influence iron metabolism, they do not impair Tf function. The study highlights the importance of a glucose-free dialysis solutions in managing anemia exacerbation in PD patients with poorly controlled anemia, potentially offering a targeted therapeutic approach to improve patient outcomes.