Postnatal Changes of Neural Stem Cells in the Mammalian Auditory Cortex

Our previous study reported neural stem cells (NSCs) in the auditory cortex (AC) of postnatal day 3 (P3) mice in vitro. It is unclear whether AC-NSCs exist in vivo. This study aims to determine the presence and changes of AC-NSCs during postnatal development and maturation both in vitro and in vivo....

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Bibliographic Details
Published in:International journal of molecular sciences 2021-02, Vol.22 (4), p.1550
Main Authors: Hu, Zhengqing, Tao, Li, Deng, Meng
Format: Article
Language:English
Subjects:
Animals
auditory cortex
Auditory Cortex - cytology
Bats
Biomarkers
Cell Differentiation
Cell Proliferation
Cells, Cultured
development
Fluorescent Antibody Technique
Gene Expression
Head injuries
Mammals
Mice
neural stem cell
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
neurosphere
postnatal
proliferation
Proteins
Statistical analysis
Stem cells
Trauma
Variance analysis
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Summary:Our previous study reported neural stem cells (NSCs) in the auditory cortex (AC) of postnatal day 3 (P3) mice in vitro. It is unclear whether AC-NSCs exist in vivo. This study aims to determine the presence and changes of AC-NSCs during postnatal development and maturation both in vitro and in vivo. P3, postnatal day 14 (P14), 2-month-old (2M), and 4-month-old (4M) mouse brain tissues were fixed and cryosectioned for NSC marker immunostaining. In vitro, P3, P14, and 2M AC tissues were dissected and cultured in suspension to study NSCs. NSC proliferation was examined by EdU incorporation and cell doubling time assays in vitro. The results show that Nestin and Sox2 double expressing NSCs were observed in the AC area from P3 to 4M in vivo, in which the number of NSCs remarkably reduced with age. In vitro, the neurosphere forming capability, cell proliferation, and percentage of Nestin and Sox2 double expressing NSCs significantly diminished with age. These results suggest that AC-NSCs exist in the mouse AC area both in vitro and in vivo, and the percentage of AC-NSCs decreases during postnatal development and maturation. The results may provide important cues for the future research of the central auditory system.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22041550